Protein-protein Interaction Network of AHR and MYCN Involved in Neuroblastoma
Date Issued
2008
Date
2008
Author(s)
Cheng, Kai-Lin
Abstract
Neuroblastoma, a heterogeneous tumor with variable clinical and biological behavior, is one of the most common pediatric cancers that derived from sympathetic nervous system. To explore the heterogeneous behavior of neuroblastoma, many prognostic biological markers have been discovered, especially v-myc myelocytomatosis viral related oncogene (MYCN) amplification predict a poor prognosis. However, the regulatory mechanism by MYCN expression still has been unclear. The microarray technique is a powerful and high-throughput method for accurately determining changes in global gene expressions. In this study, we performed microarray experiment to measure gene expression profiles of 20 Taiwanese neuroblastoma patients. Furthermore, we used pathway mapping and in silico interaction prediction to elucidate and construct the protein interaction network. We showed that 2718 genes are differentially expressed between neuroblastoma and control samples using 3-fold change as cut-off. By using unsupervised hierarchical clustering, we found that MYCN-amplified samples separated from not-amplified samples. By pathway mapping, the aryl hydrocarbon receptor (AHR) pathway was significant and MYCN and AHR have highly correlation in the expression profile. In addition, we constructed the protein network which MCYN connect through 2 nodes with AHR network and the gene expressions of MYCN- and AHR- interacted proteins are highly relevant. Our findings indicate that MYCN and AHR have highly correlation and AHR may also play an important role in neuroblastoma development. The protein-protein interaction network of AHR and MYCN involved in neuroblastoma is useful to know the regulatory mechanism by MYCN expression. This study shed light on neuroblastoma therapy.
Subjects
neuroblastoma
microarray
protein-protein interaction network
SDGs
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