Chlorophyll a, an active anti-proliferative compound of Ludwigia octovalvis, activates the CD95 (APO-1/CD95) system and AMPK pathway in 3T3-L1 cells
Journal
Food and Chemical Toxicology
Journal Volume
48
Journal Volume
48
Journal Issue
2
Journal Issue
2
Pages
716-721
Start Page
716
End Page
721
ISSN
02786915
Date Issued
2010-02
Author(s)
Abstract
Ludwigia octovalvis is an aquatic plant widely distributed in Taiwan. It is traditionally used as a diuretic and is consumed as health drink. In this study, we evaluated the anti-proliferative activity of extracts and active constituent (chlorophyll a; CHL-a) of L. octovalvis in 3T3-L1 adipocytes; its mode of action on apoptosis was also investigated. Results showed that, among the different extracts and fractions, the ethylacetate layer (EAL) possessed the most potent anti-proliferative activity. Activity guided fractionation of the EAL obtained the bioactive constituent CHL-a (IC50: 24.10 ± 0.83 nM). At concentrations 5-30 nM, CHL-a exhibited a dose-dependent accumulation of the Sub-G1 peak and caused cell cycle arrest at the G0/G1 phase. At 30 nM, it significantly reduced the cell viability, induced the appearance of DNA fragments, and enhanced the activation of caspase-3. Western blot data revealed that CHL-a decreased the level of Bcl-2, and increased the expression of CD95 (APO-1/CD95) and Bax. Furthermore, CHL-a up-regulated the AMPK and p-AMPK levels, and down-regulated the expression of PPAR-γ. These results conclude that CHL-a possesses potent anti-proliferative activity, and its apoptotic effects on 3T3-L1 adipocytes are mediated through the activation of CD95 (APO-1/CD95) system and the AMPK signaling pathway.
Subjects
3T3-L1 cells
AMPK
CD95 (APO-1/CD95)
Chlorophyll a
Ludwigia octovalvis
SDGs
Other Subjects
caspase 3; chlorophyll; Fas ligand; ludwigia octovalvis extract; mitogen activated protein kinase; plant extract; protein Bax; protein bcl 2; unclassified drug; adipocyte; animal cell; apoptosis; article; cell cycle arrest; cell cycle G0 phase; cell cycle G1 phase; cell cycle progression; cell growth; cell population; cell proliferation; cell viability; controlled study; DNA fragmentation; down regulation; enzyme activation; fractionation; gene expression regulation; Ludwigia octovalvis; medicinal plant; nonhuman; protein expression; signal transduction; upregulation; Western blotting; 3T3-L1 Cells; Adipocytes; Animals; Anti-Obesity Agents; Antigens, CD95; Apoptosis; Cell Cycle; Cell Proliferation; Chemical Fractionation; Chlorophyll; Mice; Onagraceae; Plant Extracts; PPAR gamma; Protein Kinases; Ludwigia octovalvis
Type
journal article