國人肺腺癌之基礎與臨床研究─建立體外模式研究肺腺癌轉移之機轉（III）
Date Issued
1999
Date
1999
Author(s)
楊泮池
DOI
882314B002079M39
Abstract
Lung cancer is a serious health problem
in Taiwan. The epidemiologic characteristics
such as: high female to male ratio, high
proportion of adenocarcioma, high peripheral
type lung cancer which are rather unique and
may imply that different etiologic factors
involving the pathogenesis of lung cancer in
Taiwan. This proposed project will address
on the most common cell type of lung cancer:
adenocarcinoma, especially female
adenocarcinoma and study the molecular
epidemiology, genetic alterations and
mechanism of metastasis. The subproject 4
will serve partially as a core facilities. We
will provide clinical informations, collect
tissue samples, for extraction of DNA and
RNA, blood, establish cell lines from our
own Chinese patients for the studies in other
subprojects. We also provide
immunohisto-chemical
technical support for other
collaborated laboratories. We will
particularly focus on the establishment an in
vitro model for study the mechanisms of
metastasis in lung adenocarcinoma, by
repeated selection in Transwell Membrane
Culture System, we establish high invasive
and high metastatic potential sublines.
Using these in vitro cell model, we are able
to explore the genes involving the regulation
of cancer metastasis by the technique of
differential display of gene expressions as
well as the cDNA microarray technique
Several condidate cDNAs have been
obtained which are differentially expressed
either in high invasive metastatic cell line or
in low invasive/metastatic cell line.
Northern hybridization also confirmed the
results obtained from cDNA microarray.
We cloned and transfected one candidate
gene-CRMP-1 into CL1-5. Currently we are
involved in characterization function of these genes.
Subjects
Lung adenocarcinoma
metastasis
SDGs
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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