Engineered cell-laden thermosensitive poly(N-isopropylacrylamide)-immobilized gelatin microspheres as 3D cell carriers for regenerative medicine
Journal
Materials Today Bio
Journal Volume
15
Date Issued
2022
Author(s)
Abstract
Several studies have focused on using cell carriers to solve the problem of mesenchymal stem cell expansion on regenerative medicine. However, the disadvantages of using prolonged enzymatic treatment and low cell harvest efficiency still trouble researchers. In this study, PNIPAAm-immobilized gelatin microspheres (abbreviated as GNMS) were synthesized using a simple power-driven flow-focusing microinjection system. The developed thermosensitive GNMS can allow easier harvesting of cells from the microspheres, requiring only 10 min of low-temperature treatment and 5 min of trypsin treatment. The developed GNMS was characterized by Fourier-transform infrared spectroscopy, optical microscopy, and scanning electron microscopy. Further, live/dead staining, F-actin staining, and PrestoBlue cell viability assays were used to evaluate cytotoxicity, cell morphology, cell proliferation, and harvest efficiency. The gene expression of stem cell markers was determined by real-time quantitative PCR (Q-PCR) analysis to investigate the stemness and phenotypic changes in Wharton's jelly-derived mesenchymal stem cells. The results showed that the engineered cell-laden thermosensitive GNMS could significantly increase the cell harvest rate with over 99% cell survival rate and no change in the cell phenotype. Thus, the described strategy GNMS could be the suitable 3D cell carriers in the therapeutic application and opens new avenues for regenerative medicine. © 2022 The Authors
Subjects
Cell expansion; Gelatin-PNIPAAm microspheres; Regenerative medicine; Stem cell therapy; Thermosensitive cell carriers
Other Subjects
Acrylic monomers; Amides; Cell culture; Cell proliferation; Efficiency; Expansion; Fourier transform infrared spectroscopy; Gene expression; Polymerase chain reaction; Proteins; Regenerative Medicine; Scanning electron microscopy; Stem cells; Temperature; Cell carrier; Cell expansion; Gelatin microspheres; Gelatin-PNIPAAm microsphere; Mesenchymal stem cell; PNIPAAm; Regenerative medicine; Stem cell therapy; Thermo sensitive; Thermosensitive cell carrier; Microspheres
Type
journal article