Effects of combination of garlic oil and water extract of clam on liver damage induced by carbon tetrachloride in rats
Date Issued
2007
Date
2007
Author(s)
Lin, Hui-Chen
DOI
zh-TW
Abstract
Garlic (Allium sativum L.) and clam (Corbicula fluminea Muller) are widely used as foodstuffs and traditionally used as a Chinese remedy for liver disease and chroic hepatitis. Numerous studies show that garlic oil exhibits diverse biological activities, including detoxification, antioxidation, etc. Furthermore, water extract of clam also exhibits diverse biological activities, including detoxification, antioxidation, antitumor activeity, etc. In Taiwan, the incidence of liver diseases is high, such as chronic hepatitis, liver cirrhosis and hepatocellular carcinoma which are the major causes of death. Therefore how to prevent liver disease is very important. The objective of this study was to investigate the effects of the mixture of garlic oil and water extract of clam on liver protection on the liver damage induced by carbon tetrachloride (CCl4) in rats. CCl4 is biotransformed to free radicals by the cytochrome P450 system in liver, and consequently causes lipid peroxidation of membranes that leads to liver damage. As to the acute and chronic experimental results, rats orally administered with various doses of garlic oil and water extract of clam were significantly lowered TBARS levels in liver and plasma, and decreased the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) compared with CCl4 treated groups, especially medium (1558 mg/kg BW) and high dose (3116 mg/kg BW) groups. Sample administration significantly increased glutathione (GSH) level and hepatic enzymes like glutathione peroxidease (GPx), glutathione reductase (GRd), superoxide dismutase (SOD) and catalase (CAT) activities. Rats orally administered with high dose sample were significantly lowered the concentrateion of triglyceride (TG) and total cholesterol (TC) in liver and serum, showing that garlic oil plus water extract of clam can improve lipid accumulatedion in liver. Furthermore, histopathology of liver in the sample groups showed a lower degree of inflammation and fibrosis, improving liver damage. These results indicated that the mixture of garlic oil and water extract of clam might protect hepatocytes from CCl4-induced liver damage via lowering lipid peroxidation by increasing the activities of antioxidants agents.
Subjects
大蒜精油
蜆
四氯化碳
肝損傷
麩胱甘肽
garlic oil
clam
carbon tetrachloride
liver damage
glutathione
SDGs
Type
thesis
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