Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells
Journal
2012 IEEE 6th International Conference on Nano/Molecular Medicine and Engineering
Date Issued
2012-11
Author(s)
SHIH-KANG FAN
Abstract
Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane (PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.
Type
conference paper