Studies on Cytotoxicity and Genotoxicity of Silver Particle/Silicate Platelet Nanohybrids
Date Issued
2014
Date
2014
Author(s)
Lee, Yu-Hsuan
Abstract
In recent years, silver nanoparticles (AgNP) has been used in many antibacterial products, such as drinking water purification and bone cement or treatment of many skin conditions and the use as antibacterial textile. Since nanomaterials and nanotechnologies are expected to yield numerous health and health care advances, tighter the regulation of nanotechnology has been demanded.
Silver nanoparticles (AgNP) are known for excellent bactericidal properties, but there are the concerns of the possible adversity toward normal cells. We have developed the one-nanometer-thick silicate platelets (NSP) from the natural silicate clays and employed them as the high surface support for enhancing antimicrobial efficacy but mitigating the toxicity of normal cells. The NSP nanoplatelets were in the geometric dimension of ca. 80 × 80 × 1 nm3 and possessing of intensive ionic charges on the local surface. The synthesis of the nanohybrids of AgNPs immobilized on NSP (AgNP/NSP) were proven to have the enhanced antimicrobial properties against the growth of bacteria including Gram-positive and Gram-negative. In this study, we synthesized AgNP/NSP nanohybrids in three different weight ratios (1/99, 7/93 and 15/85) and particle size in the range of 3.8-9.3 nm in diameter. Cytotoxicity by using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and genotoxicity by the methods of chromosomal aberration, comet, Ames and micronucleus assays were determined. Overall, AgNP/NSP showed none of significant cytotoxicity nor DNA damage at the bactericidal dose. By comparison, the synthesized AgNP in polymeric organic dispersants were shown high cytotoxicity and genotoxicity by the same tests. Due to the high surface support of the NSP, NSP prevents the immobilized AgNP enter cell membrane, which lower the risk associated in AgNP toxicity. Hence, the newly developed AgNP/NSP nanohybrids demonstrated low toxicity based on the cytotoxic and genotoxic tests and suitability for potential uses in biomedical areas.
Subjects
奈米混合材料
奈米銀粒子
奈米矽片
低細胞毒性
低基因毒性
抗菌
Type
thesis
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