Development of antibody functionalized magnetic nanoparticles for the immunoassay of carcinoembryonic antigen: A feasibility study for clinical use
Journal
Journal of Nanobiotechnology
Journal Volume
12
Journal Issue
1
Pages
44
Date Issued
2014
Author(s)
Abstract
Background: Magnetic nanoparticles functionalized antibodies are used for in-vitro assays on bio-markers. This work demonstrates the synthesis of high-quality magnetic nanoparticles coated with antibodies against carcinoembryonic antigen (CEA). Various characterizations, such as particle size, particle suspension, bio-activity and the stability of bio-magnetic nanoparticles suspended in liquid, are studied. The properties for the assay of CEA molecules in serum are also studied. The assay method used is so-called immunomagnetic reduction. Results: The results show that the effects of common materials in serum that interfere with detected signals are not significant. The low-detection limit is 0.21 ng/ml, which is well below the clinical threshold of 2.5 ng/ml. Conclusions: The dynamic range for the assay of CEA molecules in serum is 500 ng/ml. By assaying serum CEA molecules from 24 normal controls and 30 colorectal-cancer patients, the threshold for the serum-CEA concentration to diagnose colorectal cancer is 4.05 ng/ml, which results in a clinical sensitivity of 0.90 and specificity of 0.87. ? 2014 Yang et al.
SDGs
Other Subjects
Antibodies; Antigens; Assays; Bioassay; Body fluids; Diseases; Molecules; Nanomagnetics; Particle size; Suspensions (fluids); Synthesis (chemical); Carcinoembryonic antigen; Colorectal cancer; Common materials; Feasibility studies; Low detection limit; Magnetic nano-particles; Normal controls; Particle suspensions; Nanoparticles; antibody; carcinoembryonic antigen; magnetic nanoparticle; nanocoating; nanomaterial; antibody; carcinoembryonic antigen; magnetite nanoparticle; tumor marker; Article; biological activity; biomagnetism; cancer patient; clinical article; colorectal cancer; controlled study; feasibility study; human; immunoassay; limit of detection; liquid; molecular dynamics; molecular stability; particle size; sensitivity and specificity; signal detection; suspension; synthesis; blood; case control study; chemistry; colorectal tumor; immunoassay; immunology; procedures; temperature; Antibodies; Biomarkers, Tumor; Carcinoembryonic Antigen; Case-Control Studies; Colorectal Neoplasms; Humans; Immunoassay; Limit of Detection; Magnetite Nanoparticles; Particle Size; Temperature
Publisher
BioMed Central Ltd.
Type
journal article