The Detection of Duck Picornavirus by Reverse Transcription Polymerase Chain Reaction and Serological Survey among Bird Species in Taiwan
Date Issued
2004
Date
2004
Author(s)
Ting, Yen-Wen
DOI
zh-TW
Abstract
Duck viral hepatitis (DVH) is a high mortality disease in ducklings. Three types of DHV have been reported, and type 1 and type 3 caused by viruses belonging to Picornavidiae. In Taiwan, outbreaks of DVH were reported in 1972 and 1990, respectively. A strain of the duck pricornavirus (DPV), Tw90A, isolated in the 1990 Kaohsiung outbreak was studied. Two pairs of primers were designed based on the conserved region of 3D and were used in nested reverse transcription polymerase chain reaction (Nested-RT-PCR) to detect DPV. Day-old to 21 days-old Peking ducks without DPV antibody were inoculated with Tw90A isolate. Various organs were collected from 8 hours to 21 days after inoculation and Nested-RT-PCR was used to detect the presence of the DPV genome. The results showed that DPV persisted in the infected ducklings for at least 21 days. Most of the other organs, such as the liver, spleen, heart muscle, skeletal muscle, and kidney, became are tested positive in samples collected between 8 to 48 hours after inoculation. The detection of the DPV genome was limited to the cerebrum, esophagus, heart muscle, skeletal muscle, liver, kidney, spleen, and pancreas at 21 days post-inoculation (DPI). The weight gains of duckling inoculated with DPV were significantly lower than the un-inoculated control ducklings (p<0.01). Seroprevalence of DPV among bird species in Taiwan was also studied. The results showed positive rates of 32.95% in duck, 1.50% in chicken, 22.50% in pigeon, and 7.06% in goose. DPV positive rate was significantly higher in duck than in other species (p<0.01). Seroprevalence of vDHV and in duck were 1.1% and 62.3% respectively. DHV positive rate was significantly higher in duck than DPV and vDHV (p<0.01).
Subjects
鏈反應
鴨
反轉錄聚合酶
血清學調查
鴨小病毒
鴨病毒性肝炎
serological survey
duck virus hepatitis
duck
reverse transcription polymerase chain reaction
duck picornavirus
SDGs
Type
thesis
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