Effects of index-mismatch-induced spherical aberration on two-photon imaging in skin and tissue-like constructs
Journal
Proceedings of SPIE
Journal Volume
4963
Pages
95-104
Date Issued
2003
Date
2003
Author(s)
Tung, Chih-kuan
Periasamy, Ammasi
So, Peter T. C.
Chiu, Tsung-Kai
Lo, Wen
Wang, Po-Hsiang
Jee, Shiou-Hwa
Abstract
Index mismatch induced spherical aberration is studied by comparing imaging ability of different immersion objectives in both uniformly fluorescent solutions and fluorescently labeled skin samples using scanning two-photon fluorescence microscopy. We investigated the performances of the objectives (air, water, glycerin, and oil immersion) by measuring the fluorescence profiles at different depths. In homogeneous fluorescent samples, we found that immersion medium with compatible refractive index as the samples yields better results, and small differences in refractive indices did not cause noticeable effects. Similar results were found in skin samples. Except for the air objective, we found that the choice of immersion medium did not have significant effects for in-depth imaging in the fluorescent solutions or skin samples.
Subjects
Fluorescence; Imaging; Microscopy; Skin; Spherical aberration; Two-photon
Other Subjects
Aberrations; Biomedical engineering; Fluorescence; Microscopic examination; Multiphoton processes; Refractive index; Tissue; Two-photon fluorescence microscopy (TPFM); Medical imaging
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