A novel synthetic-genetic-array-based yeast one-hybrid system for high discovery rate and short processing time
Journal
Genome Research
Journal Volume
29
Journal Issue
8
Pages
1343-1351
Date Issued
2019
Author(s)
Yeh, Chung-Shu
Wang, Zhifeng
Miao, Fang
Ma, Hongyan
Kao, Chung-Ting
Hsu, Tzu-Shu
Yu, Jhong-He
Hung, Er-Tsi
Lin, Chia-Chang
Kuan, Chen-Yu
Tsai, Ni-Chiao
Zhou, Chenguang
Qu, Guan-Zheng
Jiang, Jing
Liu, Guifeng
Wang, Jack P.
Li, Wei
Chiang, Vincent L.
Chang, Tien-Hsien
Lin, Ying-Chung Jimmy
Abstract
targets. Yeast one-hybrid (Y1H) is one of the most extensively used methods to discover these interactions. We developed a high-throughput meiosis-directed yeast one-hybrid system using the Magic Markers of the synthetic genetic array analysis. The system has a transcription factor-DNA interaction discovery rate twice as high as the conventional diploid-mating approach and a processing time nearly one-tenth of the haploid-transformation method. The system also offers the highest accuracy in identifying TF-DNA interactions that can be authenticated in vivo by chromatin immunoprecipitation. With these unique features, this meiosis-directed Y1H system is particularly suited for constructing novel and comprehensive genome-scale gene regulatory networks for various organisms.
Type
journal article