Influence of nutritive factors on C50 carotenoids production by Haloferax mediterranei atcc 33500 with two-stage cultivation
Journal
Bioresource Technology
Journal Volume
101
Journal Volume
101
Journal Issue
16
Journal Issue
16
Pages
6487-6493
Start Page
6487
End Page
6493
ISSN
09608524
Date Issued
2010-08
Author(s)
Abstract
The production of pigments by Haloferax mediterranei ATCC 33500 with two-stage cultivation in response to nutritive factors in culture media was studied. Sodium chloride and magnesium sulfate in the second-stage media showed a marked effect upon the production of pigments, and sodium acetate could enhance the production. As the cells were harvested at mid-log phase of growth in first-stage cultivation and transferred to the defined media containing 5% sodium chloride, 0.1% sodium acetate and 8% magnesium sulfate at 37 degrees C, 120 rpm for further 24 h of cultivation, H. mediterranei exhibited to be an efficient producer of pigments. The yield of pigments could reach up to 0.604 A(494 nm) mL(-1) broth. TLC analysis and the UV-Vis spectra of individual spots thereof revealed that H. mediterranei produced three red pigments of C(50) carotenoid, namely bisanhydrobacterioruberin, monoanhydrobacterioruberin and bacterioruberin, as well as a C(45) carotenoid, 2-isopentenyl-3,4-dehydrorhodopin.
Subjects
Bacterioruberin
Carotenoids
Haloferax
Red pigments
SDGs
Publisher
Elsevier Ltd
Type
journal article