Digital Scanning Lightsheet Microscopy (DSLM) for Drosophila Whole Brain Functional Imaging
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
13303
Start Page
1330306
ISSN
16057422
ISBN (of the container)
9.78E+12
Date Issued
2025
Author(s)
Abstract
Intravital imaging has presented a powerful tool for tracking cellular function and structural dynamics in the real environment of a complex multicellular organism. However, most commercial microscopy systems are limited by optical design, allowing only long-term live imaging of transparent samples, such as Drosophila embryo, zebrafish embryo, and suffering from slow image acquisition speeds. Consequently, they can only generate two-dimensional time-lapse images at selected optical planes. Here, we introduce a high-resolution Bessel beam based vertical digital scanning light sheet microscopy (V-SPIM) for real-time imaging of the adult fruit fly brain. Compared to traditional multiphoton imaging or multi-point confocal microscopy, Bessel beam based light sheet microscope exhibits lower photodamage and faster acquisition volume rates (V.R.) higher than 1Hz (with proper exposure time (E.T.) and signal to background ratio (SNR)) for each volume(330?330?162μm), allowing us to perform long-term volumetric time-lapse functional imaging in adult animals. This novel approach allows us to visualize the olfactory coding of various odors in a multitude of cells within a single fly brain. © 2025 SPIE.
Event(s)
Neural Imaging and Sensing 2025
Subjects
Bessel beam
Drosophila Melanogaster
Lightsheet microscopy
Neuron calcium imaging
Odor preference and behavior
Olfactory system
Publisher
SPIE
Description
Neural Imaging and Sensing 2025, 27 January 2025 through 29 January 2025, San Francisco
Type
conference paper