A Novel Rare Cell Micro-Bioreactor with Reciprocated Cell Sorting, In-Situ Culturing, and Real-time Investigation
Date Issued
2006
Date
2006
Author(s)
Cheng, Tien-Ming
DOI
en-US
Abstract
In the past decades, extensive investments in basic biomolecular research have yielded about the many and complex processes involved in the development of an organism. But many questions still remain. How does a single rare cell, such as the stem cell embedded in the human tissue, give rise to a complex, multi-cellular organism? How does a rare tumor cell traveling through the human circuiting system and adhesive to a specifically targeted organ? Obviously, these questions represent a fundamental challenge in developmental biology. The demands of the new methodologies hasten the emerging technologies, such as the BioMEMS and Nanobiotechnology, trying to carry out the remained issues and obtaining the new scientific discovery.
The aim of work this thesis described is to accomplish a microfluidic based system, which is composed of a microfabricated core chip, optical instruments, and a microincubator. The subject matter is coordinated from the connection and integration of different research field, including microfabrication, microscopic optics, and cell biology. This is also the first time a microfabricated platform integrates the reciprocated sorting, in-situing culturing, long-term culture, and nanobiotechnology. In this thesis, early work is devoted on the microfabrication, and the related packaging. Reciprocated cell sorting is one of steps of a complete system. High speed sorting is achieved by the trail design and realizing of the fundamental theories of the hydrodynamics focusing and dielectrophoresis. On-chip culture is testified using several kinds of human living cells. Cytokinesises of the cancerous cells, primary cultured cells, and stem cells, are real-time monitored. The accomplished system is useful in fundamental of rare cell study, and potential in personal cell therapy.
Subjects
微機電
微製造
微流
介電泳
稀有細胞
骨髓幹細胞
細胞篩選
細胞培養
長時間監測
平台整合
MEMS
microfluidic
mFACS
hydrodynamic focusing
dielectrophoresis
cell sorting, rare cell
bone marrow stem cell
in-situing culturing
time-lapsed investigation
osteosarcoma
cytoviva
SDGs
Type
thesis
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