The study of male gametogenesis of Taiwania cryptomerioides Hayata
Date Issued
2004
Date
2004
Author(s)
陳淑華
DOI
922313B002118
Abstract
The endemic cypress species to
Taiwan, Taiwania cryptomerioides
Hayata was studies with histochemical
staining in the first year.
Over these developing stages, both
the fertile and sterile microspore did not
differ from each other in microspores
and tapetum. At mature pollen grain
(MPG) stage, lipids were still detectable
for fertile PG, but not for sterile one. It
was detectable a tremendous amount of
2
lipid granules from M to PG stages in
the locule of sterile microsporangia,
while only trace amount of fertile one.
In the fertile microspores,
polysaccharides were detectable
throughout the entire developmental
stages. In the sterile one, in contrast, less
polysaccharide was detected after
microspore stage and entirely not
detected at PG stage. In the tapetum,
polysaccharides were detectable from
the stage of sporogenous tissue to dyad
stage, either in fertile or in sterile
microsporangia. As a whole, there is no
significant difference between fertile
and sterile microsporangia in
polysaccharide content in the tapetum.
In the sterile microsporangia,
tremendous polysaccharides were
detected in the locule, while in the fertile
one none was detectable throughout the
entire developmental stages.
In the tapetum, a great amount of
proteins was found from the stage of
sporogenous tissue to that of late MMC.
At subsequent two stages, namely dyad
and tetrad stages, only a trace amount of
proteins was detected. Later than tetrad
stage, no protein was detected in the
tapetum owing to the degradation of
tapetum itself. In the protein content,
there was not significant difference
between fertile and sterile
microsporangia.
In microspores, there were also
tremendous amount of proteins
detectable from the stage of sporogenous
tissue to that of late MMC, followed by
less amount of protein content in the
subsequent developmental stages. In the
sterile microspores, less protein content
was detected at the stages from dyad to
BPG. At MPG stage, fertile microspores
were characterized by plenty of protein
contents, while sterile ones none at all.
In the sterile one, proteins were
detectable after microspore stage. In the
fertile one, on the contrary, proteins
were detected in the locule from late
microspore mother cell stage to tetrad
stage, but not at the subsequent stages.
Subjects
Taiwania cryptomerioides
male gametogenesis
male sterility
pollen development
histochemical stain
Publisher
臺北市：國立臺灣大學生命科學系
Type
report
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