─建構ECDYSONE誘導式基因表現系統以研究DNA微矩陣鑑識之基因的策略
Date Issued
2001
Date
2001
Author(s)
周祖述
DOI
892323B002010
Abstract
Regulatory expression systems, such as tetracycline repressible system, ecdysone
inducible system, and Lac operon inducible system, have been proved of tremendous value
for researchers wishing to understand the functional significance of their genes of interest. In
the post-genomic era, tight and inducible expression system is of extreme importance in the
identification of molecular targets genuine for disease pathogenesis and drug development. In
this proposal, a simple and efficient strategy is outlined to replace the traditional individual
clone-based screening of generating regulatory expression system. Taking advantage of a
GFP reporter construct under ecdysone responsive element and FACSorting based upon a
simplistic positive and negative selection scheme, we had been able to isolate population of
HEK293 and MDCK cells tightly regulated by ecdysone in term of the expression activity of
exogenously introduced gene. Our preliminary data also showed similar strategy could be
exploited to set up tetracycline inducible system. An example is provided to demonstrate
how a dual regulatory expression system, which conjugates ecdysone inducible and
tetracycline regulated systems, could be used to study the interplay of two closely related
signaling molecules.
Subjects
Ecdysone inducible system, Tetracycline regulatory expression system, and
LacSwitch expression system, Regulatory expression system
LacSwitch expression system, Regulatory expression system
Publisher
臺北市：國立臺灣大學醫學院臨床醫學研究所
Type
journal article
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