Effect of physical factors on the production of γ-decalactone by immobilized cells of Sporidiobolus salmonicolor
Resource
Process Biochemistry, 34(8), 845-850
Journal
Process Biochemistry
Pages
845-850
Date Issued
1999
Date
1999
Author(s)
Abstract
The effects of some physical factors, including preculture time, media initial pH and bead concentrations, on the production of γ-decalactone by immobilized Sporidiobolus salmonicolor CCRC 21975 within calcium alginate beads were investigated. Long-term reuse and stability of the immobilized preparation were also examined. Regardless of the preculture time, no significant difference in the production of γ-decalactone by immobilized S. salmonicolor cells was observed as long as the initial populations of the entrapped cells were the same. Calcium alginate-immobilized S. salmonicolor cells showed improved tolerance to perturbations in external pH and produced more γ-decalactone than did free cells. The patterns of changes in S. salmonicolor cells inside or outside the gel beads as well as production of γ-decalactone by immobilized S. salmonicolor did not vary significantly with bead concentration. Furthermore, after reuse of the calcium alginate- immobilized S. salmonicolor cells for 13 consecutive 4-day batch fermentations, no bead leakage was detected. The production of γ-decalactone in the 13th cycle was about 58.4% of that in the first cycle.
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Type
journal article
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