Suppression of Adipogenesis by 5-Hydroxy-3,6,7,8,3′,4′-Hexamethoxyflavone from Orange Peel in 3T3-L1 Cells
Journal
Journal of Medicinal Food
Journal Volume
19
Journal Issue
9
Pages
830-835
Date Issued
2016
Author(s)
Abstract
We reported previously that hydroxylated polymethoxyflavones (HPMFs) effectively suppressed obesity in high-fat-induced mouse. In this study, we further investigated the molecular mechanism of action of 5-hydroxy-3,6,7,8,3′,4′-hexamethoxyflavone (5-OH-HxMF), one of major HPMFs in orange peel. Treatment of 5-OH-HxMF effectively inhibited lipid accumulation by 55-60% in a dose-dependent manner. The 5-OH-HxMF attenuated adipogenesis through downregulating adipogenesis-related transcription factors such as peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding proteins (C/EBPs), as well as downstream target fatty acid synthase and acetyl-CoA carboxylase (ACC). 5-OH-HxMF activated adenosine monophosphate-activated protein kinase signaling and silent mating type information regulation 1 (SIRTUIN 1 or SIRT1) in 3T3-L1 adipocytes to decrease lipid accumulation. In addition, the inhibition rate of lipid accumulation was compared between 5-OH-HxMF and 3,5,6,7,8,3′,4′-heptamethoxyflavone (HpMF). 5-OH-HxMF inhibited lipid accumulation 15-20% more than HpMF did, indicating that hydroxyl group at position 5 can be a key factor in the suppression of adipogenesis. ? Copyright 2016, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition 2016.
Subjects
3T3-L1; 5-hydroxy-3,6,7,8,30,40-hexamethoxyflavone; Adipogenesis; Medicinal food; Orange peel
SDGs
Other Subjects
5 hydroxy 3,6,7,8,3',4' hexamethoxyflavone; acetyl coenzyme A carboxylase; CCAAT enhancer binding protein; fatty acid synthase; hydroxymethylglutaryl coenzyme A reductase kinase; lipid; peroxisome proliferator activated receptor gamma; plant extract; sirtuin 1; unclassified drug; 3,3',4',5,6,7,8-heptamethoxyflavone; 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone; acetyl coenzyme A carboxylase; adenosine phosphate; antiobesity agent; CCAAT enhancer binding protein alpha; flavone derivative; flavonoid; peroxisome proliferator activated receptor gamma; plant extract; protein kinase; sirtuin 1; 3T3 cell line; adipocyte; adipogenesis; animal cell; Article; cell viability; controlled study; cytotoxicity; down regulation; drug mechanism; drug structure; lipid storage; mouse; nonhuman; obesity; orange (fruit); priority journal; signal transduction; 3T3-L1 cell line; adipogenesis; adipose tissue; animal; chemistry; Citrus; drug effect; fruit; lipid metabolism; metabolism; obesity; phytotherapy; 3T3-L1 Cells; Acetyl-CoA Carboxylase; Adenosine Monophosphate; Adipocytes; Adipogenesis; Adipose Tissue; Animals; Anti-Obesity Agents; CCAAT-Enhancer-Binding Protein-alpha; Citrus; Flavones; Flavonoids; Fruit; Lipid Metabolism; Mice; Obesity; Phytotherapy; Plant Extracts; PPAR gamma; Protein Kinases; Signal Transduction; Sirtuin 1
Type
journal article