奈米液滴產生器計劃-子計畫一:創新之奈米螢光噴射液滴研究 與近場全反射量測
Date Issued
2004-07-31
Date
2004-07-31
Author(s)
黃榮山
DOI
922212E002088
Abstract
TIRF microscopy is a well-suited
technique for real-time imaging and
monitoring of a single protein molecule in
nano-layer fluidics due to its unique
evanencent wave at the optically
index-mismatch interaface that may excites
fluorescences at the transparent near-wall
region. Recent advances in charge coupled
device (CCD) camera detection efficiency
and speed have enabled the microscopy of
temporal and spatial resolutions to be
far-reaching 0.033 s and 0.3 μm,
respectively. Based on the fluorescent beads
analysis, which are 1.1 μm in size, the
capability of TIRFM for single molecules
monitoring and tracking, even the
measurement in lateral size of molecules
was demonstrated.
Publisher
臺北市：國立臺灣大學應用力學研究所
Type
report
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