CCT chaperonin在線蟲早期胚胎發育的功能分析
Functional analysis of CCT chaperonin in early embryogenesis in Caenorhabditis elegans
Date Issued
2006
Date
2006
Author(s)
Chiu, Min-Jan
DOI
zh-TW
Abstract
Eukayotic chaperonin CCT (Chaperonin contain T-complex polypeptide also named TriC)，established by 8 subunits，is a 16- mer double-ring complex. CCT contributes to the folding of newly synthesized proteins and the refolding of denatured proteins. Actin, tubulin and several other proteins are well known activated by CCT folding. New proteins are still found to interact with CCT, and many of them WD repeat proteins. CCT subunits are different in protein sequences, suggesting specification between them. We knockdown the subunits in Caenorhabditis elegans and found them all essential for early embryogenesis. We found defects in spindle fiber formation，chromosome segregation，cytokinesis and others，indicating a pleiotropic effect. Our observation also suggests deficiency in tubulin. However, the progeny arrest in larval stage with degenerate gonads and intestinal lumens enlarged by undigested bacteria. And they are sensitive to mechanical pressure. Similar phenotypes are in let-363 (CeTOR) mutant. TOR is the central controller of cell growth. We suggest a role of CCT in cell growth and metabolism.
Subjects
線蟲
細胞骨架
胚胎發育
細胞生長
細胞週期
C. elegans
CCT
Chaperonin
TOR
tubulin
embryogenesis
RNA intreference
WD repeat
cell growth
Type
other