Identification of novel peptides targeting to oral squamous cell carcinoma in Taiwan
Date Issued
2005
Date
2005
Author(s)
Huang, Chia-Ting
DOI
en-US
Abstract
In Taiwan, oral cancer is the fourth leading cause of cancer death in male. Approximately 80% of all oral cancer deaths are associated with the areca quid chewing habit. Despite significant improvements in diagnosis, local management, and chemotherapy of head and neck cancer, there is no significant increase in long-term survival rates over the past 30 years. To improve the survival rate, we identify several 12-mer peptides specifically binding to a Taiwanese oral squamous cell carcinoma (OSCC) TW2.6 with a phage displayed random peptide library. The selected phage clones and synthetic phage-derived peptides specifically bound to the cell surfaces of TW2.6 cells and a Taiwanese oral dysplasia cell line TW1.5, but not normal oral keratinocyes. In SCID mice bearing OSCC xenografts, the selected phage clones specifically bound to the tumor mass and the effects was inhibited by competition with phage-derived peptides. Among all the selected phage clones, C39-phage showed the highest targeting ability in tumor mass with 27.78-fold increase in concentration compared to control phage. Furthermore, C39-peptide-linked liposomes that carried doxorubicin (C39-peptide-Lipo-Dox) not only suppressed tumor growth better than Lipo-Dox but also showed no significant body weight change. These results indicate that the novel peptides specifically binding to OSCC cells and a good candidate for targeted drug delivery to OSCC solid tumors.
Subjects
噬菌體顯現法
口腔癌
胜肽
phage display
oral cancer
peptide
SDGs
Type
other
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