Study of Phylogenetic Relationship and Environmental Adaption in the Genus Isoetes in Taiwan
Date Issued
2009
Date
2009
Author(s)
Chen, Yan-Huey
Abstract
Isoetes sp. is an ancient plant since Late Devonia. There only one I. sp. had been named I. taiwanensis DeVol in Taiwan. However, there was found another habitat of I. sp. at the Taiwu mountain in Kinmen. In the pass research, the characteristics of quillwort in Kinmen are similar to I. taiwanensis, but still have some differences between two of them. And the internally transcribed spacers(ITS) region and the second intron of LEAFY homolog are not proper to be the molecular marker on distinguishing the phylogeny of the quillwort in Kinmen. Besides, the range of habitat in Kinmen is narrow, and the amount of quillwort is few. The aim to discuss the phylogeny of this quillwort, RAPD and small heat shock protein sHSP21 was used in this study. The RAPD analysis also showed only 31.42% similarity between I. taiwanensis and the quillwort in Kinmen. And by amplifying the sHSP21 partial conserved sequence of I. taiwanensis and the quillwort in Kinmen with Polymerase Chain Reaction, it was found 96.7％ similarity between them. In addition, the sHSP21 mRNA expression and spore germination as well as temperature response varies between these two samples. And the data in this thesis showed the genetic diversity of the quillwort group at Kinmen habitat is very low and this species of quillwort is endangered.
Subjects
molecular maker
genetic diversity
Random Amplified Polymorphic DNA(RAPD)
Type
thesis
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