Microcystin-LR Inhibits zebrafish embryonic development
Date Issued
2005
Date
2005
Author(s)
Wang, Pei-Jen
DOI
zh-TW
Abstract
Microcystin-LR（MC-LR）, a cyanobacterial toxin, is a potent inhibitor of protein phosphatase 1（PP1）and protein phosphatase 2A（PP2A）. PP1 and PP2A are critical regulators in embryonic development. However, the effects of MC-LR in embryonic development are controversial. MC-LR has been demonstrated to be highly toxic in medaka, but not in zebrafish or rabbit embryos. The causes of difference may be due to membrane impermeability that impaired the delivery of MC-LR into cytoplasm of zebrafish and rabbit embryos. Therefore, we microinjected MC-LR directly into the developing zebrafish embryos and investigated the effects of MC-LR on embryonic development. We demonstrated that MC-LR induced the lethality of zebrafish embryos in a dose- and time-dependent manner. MC-LR also induced the loss of blastomere coherence via the interference of β-catenin and cadherins distributions. Furthermore, the MC-LR treated fry revealed various developmental defects. These results suggested that MC-LR might affect the phosphorylation equilibrium of signaling molecules, including β-catenin and cadherins, required early in zebrafish embryonic development.
Subjects
β-catenin和cadherin
胚胎
蛋白質去磷酸酶
1,2A
肌動蛋白
微囊藻毒
斑馬魚
actin
Embryos
Zebrafish
PP2A
PP1
β-catenin and cadherin
Microcystin
Type
other
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