Identification of differentially expressed genes and demonstration of long-duration muscle dedifferentiation during limb regeneration in axolotls
Date Issued
2015
Date
2015
Author(s)
Wu, Cheng-Han
Abstract
Urodele amphibians have an extraordinary ability to regenerate lost limbs. Amputation of the limb of axolotl causes an initial wound healing response followed by blastema formation and the regeneration of the new limb. In initial stage of limb regeneration, blastemal cells are induced by nerves and the apical epithelial cap. Nerve functions have long been the focus of limb regeneration studies. Although considerable progress has been made in inquiring into the nerve-dependency of salamander, a broader systemic perspective is still needed to investigate the underlying mechanisms upon the presence of a nerve. In this study, a high-throughput sequencing approach was used to compare transcript abundance among regenerating limbs, and limbs denervated at the time of amputation. This result addressed the biological processes which are associated with blastemal formation and nerve-dependency, and will greatly assist future research of limb regeneration in axolotl. Furthermore, I added evidence that dedifferentiation persisted from mid-bud to late differentiation stage by showing expression of Pax7 transcripts in the remnant muscle fiber ends. Moreover, in vivo diffusion tensor imaging and stained tissue sections indicate a gap between the regenerating and parental muscles. Taken together, the present data suggested that prolonged dedifferentiation at remnant muscle ends might facilitate accurate connection between regenerating and parental muscle fibers due to their immature status. Additionally, these results may assist in the understanding of limb regeneration, ultimately facilitating the development of regenerative medicine in mammals.
Subjects
blastemal
limb regeneration
nerve-dependency
axolotl
dedifferentiation
muscle
diffusion tensor imaging
regenerative medicine
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-104-D97642006-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):c6f87cabe8d4ed2d0a1e54824c3027be