MUC20 promotes aggressive phenotypes of epithelial ovarian cancer cells via activation of the integrin β1 pathway
Journal
Gynecologic Oncology
Journal Volume
140
Journal Issue
1
Pages
131-137
Date Issued
2016
Author(s)
Shyu M.-K.
Chou C.-H.
Huang M.-J.
Lin T.-C.
Chen S.-T.
Abstract
Objective Mucin (MUC) 20 has recently been implicated to play a role in human carcinogenesis. However, the role of MUC20 in epithelial ovarian cancer (EOC) remains to be elucidated. Methods MUC20 expression was assessed in tissue microarray and tumor specimens of EOC patients by immunohistochemistry. Effects of MUC20 on cell viability, adhesion, migration, and invasion were analyzed in MUC20 overexpressing or knockdown EOC cells. Western blotting was performed to analyze signaling pathways modulated by MUC20. Results MUC20 was overexpressed in EOC samples compared with benign tissues. High MUC20 expression was significantly associated with poor overall survival in patients with advanced-stage disease. MUC20 overexpression significantly enhanced EOC cell migration and invasion, but not viability. Mechanistic investigations showed that MUC20 increased cell adhesion to extracellular matrix (ECM) proteins and enhanced activation of integrin β1 and phosphorylation of focal adhesion kinase (FAK). The enhancement of cell motility and the integrin β1 signaling by MUC20 was significantly suppressed by integrin β1 blocking antibody. Furthermore, these effects of MUC20 on EOC cells were also demonstrated in MUC20 knockdown cells. Conclusions Our results suggest that MUC20 enhances aggressive behaviors of EOC cells by activating integrin β1 signaling and provide novel insights into the role of MUC20 in ovarian cancer metastasis. ? 2015 Elsevier Inc. All rights reserved.
SDGs
Other Subjects
beta1 integrin; blocking antibody; collagen; collagen type 1; collagen type 4; fibronectin; focal adhesion kinase; laminin; mucin; mucin 20; scleroprotein; unclassified drug; beta1 integrin; MUC20 protein, human; mucin; advanced cancer; aggression; Article; cancer staging; cancer survival; carcinogenesis; cell adhesion; cell function; cell invasion; cell migration; cell motility; cell viability; clinical assessment; clinical evaluation; comparative study; controlled study; disease association; enzyme activation; female; gene overexpression; genetic transfection; human; human cell; immunohistochemistry; major clinical study; ovary carcinoma; overall survival; phenotypic variation; priority journal; protein expression; protein function; protein phosphorylation; signal transduction; tissue microarray; Western blotting; biosynthesis; extracellular matrix; gene silencing; genetics; metabolism; neoplasm; ovary tumor; pathology; phenotype; physiology; tumor cell line; Antigens, CD29; Cell Adhesion; Cell Line, Tumor; Extracellular Matrix; Female; Gene Knockdown Techniques; Humans; Mucins; Neoplasms, Glandular and Epithelial; Ovarian Neoplasms; Phenotype; Signal Transduction
Publisher
Academic Press Inc.
Type
journal article