https://scholars.lib.ntu.edu.tw/handle/123456789/109751
Title: | EFFECTS OF GLYCOLIC ACID ON THE INDUCTION OF APOPTOSIS VIA CASPASE-3 ACTIVATION IN HUMAN LEUKEMIA CELL LINE (HL-60) | Authors: | YANG, JEN-HUNG CHOU, CHI-CHUNG CHENG, YA-WEN SHEEN, LEE-YAN 鄭雅文 |
Keywords: | glycolic acid;human leukemia HL-60 cells;apoptosis;flow cytometry | Issue Date: | 2004 | Journal Volume: | v.42 | Journal Issue: | n.11 | Start page/Pages: | 1777-1784 | Source: | FOOD AND CHEMICAL TOXICOLOGY | Abstract: | Apoptosis is a particular process that leads to the programmed cell death, and it has been a potentially therapeutic target of cancer. In this study, we evaluated the possible apoptotic effects of glycolic acid on human leukemia cell line (HL-60) in vitro. The morphological changes, cell viability, apoptosis induction, and caspase-3 activity were measured by phase microscopy, flow cytometry, and Western blot analysis. Morphological changes including shrinkage of cells were clearly demonstrated in HL-60 cells treated with increasing concentrations of glycolic acid. Cell viability was significantly affected by glycolic acid treatment in a dose- and time-dependent manner. In comparison to the control group, glycolic acid treatment had a profound effect in the induction of apoptosis by flow cytometric assays. In the cell cycle analysis, glycolic acid caused the increased percentage of cells in G2/M phase and the decreased expression of the cyclin A and cyclin B1, suggesting the induction of G2/M arrest of cell cycle by glycolic acid. Moreover, glycolic acid treatment promoted caspase-9 and -3 activity in a dose-dependent manner, but caspse-8 activity was not affected during the same process. Glycolic acid co-administrated with broad-spectrum caspase inhibitor, z-VAD-fmk, caspase-3 activity was blunted and apoptosis was also markedly blocked in HL-60 cells. In conclusion, glycolic acid-induced apoptosis in HL-60 cells may be through the activation of caspase-3. Future studies focusing on cell signaling and biological significance of glycolic acid-induced apoptosis would lead to exploring the mechanisms of chemotherapeutic potency of glycolic acid in human cancers. (C) 2004 Elsevier Ltd. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/81331 |
Appears in Collections: | 健康政策與管理研究所 |
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