https://scholars.lib.ntu.edu.tw/handle/123456789/110908
Title: | 環境職業生殖危害(V)—液晶顯示器製造女性員工生殖內分泌研究 | Authors: | 陳保中 | Keywords: | 雌激素;促濾泡成熟激素;不孕症;液晶螢幕顯示器製造;黃體激素;月經;estrogens;follicle stimulating hormone;infertility;female;liquid crystal display manufacturing;luteinizing hormone;menstruation;progesterone | Issue Date: | 31-Jul-2005 | Publisher: | 臺北市:國立臺灣大學公共衛生學院職業醫學與工業衛生研究所 | Abstract: | 在國際上已有許多的研究,利用偵測每天尿中荷爾蒙代謝物來評估女性生殖健康及生 育能力。在先前的研究中,已利用偵測每天尿中荷爾蒙代謝物的方法証明職場上的壓 力、抽菸、喝酒及職業方面對生殖系統上的影響。利用此評估方法,我們可以用來測 量月經週期長度、濾泡期長度、黃體期長度、排卵狀況以及荷爾蒙濃度的差異,藉此 我們可以評斷暴露物質危害標的器官。 本研究為一橫斷性研究,對國內液晶顯示器製造廠做一生殖內分泌的研究,評估液晶 顯示器製造業女性員工職場暴露對生殖系統的影響。月經功能是以問卷調查來量測。 生殖內分泌方面,除了問卷資料及日誌並利用每天收集的尿液,偵測尿中荷爾蒙代謝 物來評估。在暴露評估方面,我們使用直讀式儀器針對無塵室三個區域做一個全面性 的偵測,並利用揮發性有機化合物不鏽鋼筒被動採樣作24 小時空氣採樣。並針對整個 廠區,距離所有機台及設備30 公分處作一電磁場強度的測量。 於2003 年10 月開始進行問卷調查,總共有256 女性員工完成問卷我們利用相關資料 篩選出合適的研究對象加入後續的生殖內分泌研究當中。除了已停經、懷孕、正哺餵 母乳及,有112 位女性員工同意加入生殖內分泌研究。另外,研究對象會收集每天起 床後的第一泡尿液並收集日誌,為期約1.5 個月經週期,於2003 年10 月至2004 年4 月間完成。利用偵測尿中荷爾蒙代謝物以評估其月經功能及生育力。促濾泡成熟激素 (follicle-stimulating hormone, FSH )、雌激素(estrogen )及黃體激素(progesterone ) 為我們主要探討的荷爾蒙物質。尿中FSH 、estrogen 及progesterone 之代謝物以酵素連 結免疫吸附分析法(enzyme-linked immunosorbent assay, ELISA )來分析。之後利用分 析日誌中月經來潮的資料及尿液中荷爾蒙代謝物質的濃度,評估月經週期中濾泡期及 黃體期的長短、月經週期是否排卵及荷爾蒙濃度的差異。 結果顯示,模組區的女性員工於濾泡前期,尿中促濾泡成熟激素及雌激素的代謝物濃 度比起其他暴露組來得高。於線性迴歸分析中,相對於面板區,模組區的女性員工於 早期濾泡期,尿中促濾泡成熟激素及雌激素的代謝物濃度較高(β =0.08, 95% CI = 0.00, 0.16;β =2.38, 95% CI = 0.25, 4.51)。由此結果推論,模組區女姓員工暴露於較高濃度 多重化學物質,造成卵巢功能低落。因為卵巢功能低落,需要更多促濾泡成熟激素刺 激濾泡成熟。而促濾泡成熟激素在濾泡前期主要刺激濾泡中細胞分泌雌激素,所以較 高濃度的促濾泡成熟激素亦會帶動雌激素的上升。 Assays of prospectively collected daily urine samples for metabolites of reproductive endocrine such as follicle stimulating hormone (FSH), estrogens, and progesterone have been used in much epidemiologic studies to assess ovulatory status, timing of ovulation and menstrual function. Previous epidemiologic studies have examined the effects of phychological stress in work place, smoking, and occupation on menstrual function by using daily urinary hormone metabolites. In this study, we used this tool to estimate the length of segment, ovulatory status, and hormone levels. By the assessment, we could determine the target organs of the occupational exposure. The objective of this cross-sectional study was to assess the potential reproductive endocrine effects of occupational exposure in the LCD manufacturing. Menstrual function was estimated by questionnaire. Reproductive endocrine was evaluated by detecting the concentration of reproductive hormonal metabolites in urine, either questionnaire or daily diary. Furthermore, we used handheld volatile organic compound (VOC) monitor and 24 hours canister sampling to assess potential chemical exposure. Electric and magnetic field exposure data were collected from an EMDEX meter. The study population consisted of female employees in a LCD plant in Taiwan. About 256 female workers have completed the questionnaire during the end of 2003. They were screened by a face-to-face interview to identify those who were eligible and willing to collect and freeze urine samples daily after waking for up to 1.5 menstrual cycles. Finally, urine samples of 96 subjects were included in urinary hormone analysis. Participants completed a detailed baseline questionnaire. We assayed FSH, E1C (estrone conjugates) and PdG (pregnanediol-3-glucuronide) by ELISA. We also obtained reproductive and exposure information from baseline questionnaire and daily diary. After adjusting effects of factors on hormonal excretion, E1C level of the female workers in module group still had a significantly increased (2.38 ng/mg Cr, 95% CI: 0.25, 4.51) compared with the female workers in panel group in early follicular phase. FSH level of the women in module group had a little significantly increased (0.08 mIU/mg Cr, 95% CI: 0.00, 0.16) adjusted by effects compared with the women in panel group in early follicular phase. The possibility is that multiple chemical exposures may diminish ovarian oocyte reserve or induced ovarian failure. Furthermore, shorten follicular phase may lead to shorten menstrual cycle. It is consistent with the finding of our previous study. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/4985 | Other Identifiers: | 932320B002088 | Rights: | 國立臺灣大學公共衛生學院職業醫學與工業衛生研究所 |
Appears in Collections: | 環境與職業健康科學研究所 |
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932320B002088.pdf | 207.57 kB | Adobe PDF | View/Open |
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