Molecular Epidemiology of Human Papillomavirus and Cervical Cancer
Date Issued
2005
Date
2005
Author(s)
Chen, Yi-Chu
DOI
zh-TW
Abstract
Abstract
Part I: Type-specific prevalence and determinants of Human papillomavirus infection among women in Taiwan
Background：Infection with Human papillomavirus (HPV) is a necessary cause of cervical cancer. However, there were few studies to investigate the prevalence and determinants of HPV infection among women in Taiwan.
Methods：In this cross-sectional survey, we enrolled 5487 women aged 30-64 years old from seven townships in Taiwan. Cervical cells collected at enrollment were tested for HPV genotypes by polymerase chain reactions and genechip hybridization. Logistic regression models were used to estimate odds ratio (OR) with its 95% confidence interval (CI) for each determinant of HPV infection.
Result：The overall age-adjusted prevalence of HPV infection was 12.9%. Prevalence of HPV infection was significantly associated with the age at first sexual intercourse, number of lifetime sexual partners, use of intrauterine device, use of condom and body mass index.
Conclusions：The prevalence of HPV infection is relatively high among women in Taiwan. As most determinants of HPV infection are related to sexual behavior, comprehensive health education on safe sexual activities is highly recommended.
Part II: Associations between cervical cancer and biomarkers of HPV type 16 infection
Background：HPV type 16 (HPV 16) was the most important high risk type of HPV. Biomarkers of HPV 16 infection include HPV DNA in cervical cells and antibodies against HPV 16 (anti-HPV 16) in serum, but there were few studies to investigate the combined effects of these two biomarkers on cervical cancer.
Methods：This nested case control study included 43 cases of newly diagnosed cervical cancer (including carcinoma in situ and invasive carcinoma) and 200 unaffected controls matched with cases on age, residential township and date at enrollment. Cervical cells collected at enrollment were tested for HPV genotypes by polymerase chain reactions and genechip hybridization. Anti-HPV 16 was tested by enzyme-linked immunoassay. Conditional logistic regression models were used to estimate odds ratio (OR) with its 95% confidence interval (CI) for these two biomarkers separately and jointly.
Results：A significantly increased risk of cervical cancer was associated with the positivity of HPV DNA and anti-HPV 16 showing OR (95% CI) of 25.0 (6.9-91.5) and 6.3 (1.3-31.6), respectively. Educational level and use of oral contraceptive were significantly associated with cervical cancer after adjustment for the infection of all high risk types of HPV.
Conclusions：Positivity of both HPV 16 DNA in cervical cells and anti-HPV 16 in serum are important risk factors for cervical cancer.
Subjects
人類乳突病毒
子宮頸癌
盛行率
決定因子
人類乳突病毒16型
HPV
Cervical cancer
Prevalence
Determinants
HPV 16
SDGs
Type
thesis
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