DETERMINANTS OF FORMATION OF AFLATOXIN-ALBUMIN ADDUCTS: A SEVEN- TOWNSHIP STUDY IN TAIWAN

Abstract

Dietary exposure to aflatoxins is one of the major risk factors for hepatocellular carcinoma. Individual susceptibility to aflatoxin-induced hepatocarcinogenesis may be modulated by both genetic and environmental factors affecting metabolism, A cross-sectional study was performed to evaluate determinants of the formation of aflatoxin covalently bound to albumin (AFB(1)-albumin adducts). A total of 474 subjects who were free of liver cancer and cirrhosis and were initially selected as controls for previous case-control studies of aflatoxin-induced hepatocarcinogenesis in Taiwan, were employed in this study. Aflatoxin-albumin adducts were determined by competitive enzyme-linked immunosorbent assay, hepatitis B surface antigen and antibodies to hepatitis C virus by enzyme immunoassay, as well as genotypes of glutathione S- transferase M1-1 and T1-1 by polymerase chain reaction. The detection rate of AFB(1)-albumin adducts was significantly higher in males (42.5%) than in females (21.6%) ( multivariate-adjusted odds ratio=2.6, 95% confidence interval = 1.4-5.0). The formation of detectable albumin adducts was moderately higher in hepatitis B surface antigen carriers (42.8%) than in non-carriers (36.6%) (multivariate -adjusted odds ratio = 1.4, 95% confidence interval = 1.0-2 . 1). In addition, the detection rate of AFB(1)-albumin adducts tended to increase with the increasing number of null genotypes of glutathione S-tronsferose M1-1 and glutothione S-transferase T1-1. In conclusion, this cross- sectional study has assessed the relative contributions of environmental exposure and host susceptibility factors in the formation of AFB(1)-albumin adducts in a well characterised Chinese adult population. This study further emphasises the necessity to reduce aflatoxin exposure in people living in an area endemic for chronic hepatitis B virus infection. (C) 2002 Cancer Research UK.