https://scholars.lib.ntu.edu.tw/handle/123456789/112664
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 流行病學研究所;Graduate Institute of Epidemiology | en |
dc.contributor.author | CHIOU, SHYAN-SONG | en |
dc.contributor.author | CHEN, WEI-JANE | en |
dc.contributor.author | 陳為堅 | zh-tw |
dc.creator | CHIOU, SHYAN-SONG;CHEN, WEI-JANE | en |
dc.creator | 邱賢松;陳為堅 | zh-tw |
dc.date | 2003 | en |
dc.date.accessioned | 2008-10-06T02:36:36Z | - |
dc.date.accessioned | 2018-06-29T17:59:31Z | - |
dc.date.available | 2008-10-06T02:36:36Z | - |
dc.date.available | 2018-06-29T17:59:31Z | - |
dc.date.issued | 2003 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/82087 | - |
dc.description.abstract | Japanese encephalitis (JE) is an important mosquito-borne viral disease in Southeast Asia. Isolation of JE virus from peripheral blood is usually difficult because of transient and low titer of viremia. An in situ reverse transcriptase- polymerase chain reaction (RT-PCR) method was designed to amplify gene (envelope) fragments of JE virus residing in peripheral blood mononuclear cells (PBMCs) without extraction of RNA. Baby hamster kidney-21 cells infected with the T1P1 strain of JE virus (an isolate from Armigeres subalbatus collected in Taiwan) were fixed with 4% par formaldehyde and permeabilized with 0.1% Triton X-100. The RT-PCR was then performed in microtubes using digoxigenin- labeled primers. Virus-positive PBMCs were detected in mice at day 1 and day 3, but not day 5, after intravenous inoculation with JE virus, suggesting that detectable virus circulating in the blood of mice is present for only 2-3 days. On examination of mouse brain tissues, viral RNAs were absent until day 3 post-inoculation. This implied that virus migration from the peripheral blood into the central nervous system occurs at or after day 3 post-inoculation. This method is unique in that the reactions can be conducted in tubes; this makes it convenient, accurate, and efficient compared with the conventional in situ RT-PCR on slides. | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE v.69 n.6 pp.648-651 | en |
dc.relation.ispartof | AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | - |
dc.title | SHORT REPORT: DETECTION OF JAPANESE ENCEPHALITIS VIRUS IN MOUSE PERIPHERAL BLOOD MONONUCLEAR CELLS USING AN IN SITU REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION | en |
dc.type | journal article | en |
dc.relation.pages | 648-651 | - |
dc.relation.journalvolume | v.69 | - |
dc.relation.journalissue | n.6 | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en_US | - |
顯示於: | 流行病學與預防醫學研究所 |
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