https://scholars.lib.ntu.edu.tw/handle/123456789/112670
標題: | CYTOCHROME P4502E1 GENETIC POLYMORPHISMS AND RISK OF NASOPHARYNGEAL CARCINOMA: RESULTS FROM A CASE-CONTROL STUDY CONDUCTED IN TAIWAN | 作者: | CHEN, CHIEN-JEN CHENG, YU-JUEN HSU, MOW-MING |
公開日期: | 1995 | 起(迄)頁: | - | 來源出版物: | CANCER EPIDEMIOLOGY, BIOMARKERS AND PREVENTION v.4 pp.607-10 | 摘要: | CYP2E1 is responsible for the metabolic activation of nitrosamines believed to be involved in the pathogenesis of various tumors. Nasopharyngeal carcinoma (NPC) is a tumor thought to be linked to nitrosamine exposure. To investigate the possible role of CYP2E1 genetic polymorphisms in the etiology of this tumor, we inverstigated 50 histologically confirmed NPC cases from Taiwan and 50 controls matched to cases on age, sex, and residence. Samples were examined for RFLPs in the CYP2E1 gene by PCR amplification followed by digestion with DraI and RsaI. Among healthy controls, the allelic frequency of wild-type and variant forms of CYP2E1 were 79 and 21%, respectively, using Dral enzyme digestion and 82 and 18%, respectively, using RsaI enzyme digestion. As compared with individuals who were homozygous for the wild- type CYP2E1 gene, those found to be homozygous for the variant form of the gene by DraI digestion were at a 5-fold excess risk of disease (95% confidence interval=0.95-16). Similarly, subjects homozygous for the variant form of the CYP2E1 gene by RsaI dugestion were at 7.7-fold excess risk of developing NPC (95% confidence interval = 0.87- 68). Individuals found to be heterozygous for the gene were at similar risk of disease compared to those homozygous for the wild-type gene. A strong association was observed between the RFLPs detected by DraI and RsaI digestion of CYP2E1 ; a correlation coefficient of 0.86 for controls and 0.91 for cases was observed. Interestingly, all individuals with the variant form of CYP2E1 detected by RsaI enzyme digestion also exhibited the variant form of the gene using Dral enzyme digestion, although the reverse was not always true. Our results confirm previous findings suggesting that the distribution of CYP genotypes among Oriental populations varies from that observed among Caucasians and demonstrate for the first time a possible association between CYP2E1 genetic polymorphisms and the risk of developing NPC. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/82095 |
顯示於: | 流行病學與預防醫學研究所 |
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