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  4. QUALITY ASSURANCE OF GENOTYPING ARRAY FOR DETECTION AND TYPING OF HUMAN PAPILLOMAVIRUS
 
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QUALITY ASSURANCE OF GENOTYPING ARRAY FOR DETECTION AND TYPING OF HUMAN PAPILLOMAVIRUS

Resource
JOURNAL OF VIROLOGICAL METHODS,140(1-2),1-9.
Journal
Journal of Virological Methods
Pages
1
Date Issued
2007
Date
2007
Author(s)
林景堉
陳慧祺
游山林
游秋綿
李玫萱
周雨青
陳建仁
DOI
10.1016/j.jviromet.2006.10.004
URI
http://ntur.lib.ntu.edu.tw//handle/246246/210496
Abstract
The aim of this study was to evaluate the sensitivity, specificity, reliability and reproducibility of the EasyChip? HPV blot for human papillomavirus (HPV) genotyping. Type-specific sensitivity and specificity for 39 types of HPV (HPV 6, 11, 16, 18, 26, 31, 32, 33, 35, 37, 39, 42, 43, 44, 45, 51, 52, 53, 54, 55, 56, 58, 59, 61, 62, 66, 67, 68, 69, 70, 72, 74, 82, CP8061, CP8304, L1AE5, MM4, MM7 and MM8) were examined. The operating environment, reliability, reproducibility and blot interpretation were assessed by a quality assurance system. Each batch experiment contained samples from 89 cervical specimens and 7 extrinsic controls. Caski, HeLa and Jurkat cells, male human blood cell DNA and sterile water were used to assess reliability. Furthermore, pairs of sibling controls were used to assess reproducibility. The overall sensitivity of HPV detection was 1-50 copies of HPV genome equivalent. There was no cross-reactivity with amplicons of other HPV genotypes. One hundred batch experiments demonstrated that the reliability was excellent. The intra-batch and inter-batch reproducibility was 98 and 97%, respectively. It was concluded that the EasyChip? HPV blot is a highly sensitive, reliable and reproducible tool for detection and identification of HPV genotypes. ? 2006 Elsevier B.V. All rights reserved.
Subjects
Human papillomavirus; Reliability; Reproducibility; Sensitivity; Specificity
SDGs

[SDGs]SDG3

Other Subjects
DNA; water; article; blood cell; blotting; cancer cell; Caski cell; controlled study; cross reaction; gene amplification; genotype; HeLa cell; human; human cell; leukemia cell line; priority journal; quality control; reliability; reproducibility; sensitivity and specificity; virus detection; virus gene; virus genome; virus identification; Wart virus; Human papillomavirus
Type
journal article

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