https://scholars.lib.ntu.edu.tw/handle/123456789/114526
標題: | DETERMINATION OF AFLATOXIN M1 IN MILK AND MILK POWDER USING HIGH-FLOW SOLID-PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY | 作者: | CHEN, CHIA-YANG ���a�� |
關鍵字: | certified reference material;multifunctional cleanup column;Immunoaffinity column;LC-MS/MS;solid-phase extraction;aflatoxin M1 | 公開日期: | 2005 | 卷: | v.53 | 期: | n.22 | 起(迄)頁: | 847-4 | 來源出版物: | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 摘要: | Animal feeds occasionally have some degree of contamination by Aspergillus spp. Even pasteurized milk at times contains the toxic liver carcinogen aflatoxin M1 (AFM1). Confirmation of its presence is now done with solid-phase extraction ( SPE) and high-performance liquid chromatography (HPLC)- fluorescence, using a small enough sample that SPE time is reasonable. In this study 200 mL of milk was extracted using a C18 disk at a flow rate of ~100 mL/min and AFM1 quantified by HPLC-tandem mass spectrometry with negative electrospray ionization. The effectiveness and cleanup efficacy of immunoaffinity columns (IAC) was compared with that of Mycosep multifunctional cleanup columns (MFC). Average recovery and detection limits of whole milk and low- fat milk cleaned up by IAC were significantly superior to those obtained with the MFC (78-87 % and 0.59-0.66 ng/L, respectively). The new procedure improves extraction speed, sensitivity, and specificity. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/81720 |
顯示於: | 環境與職業健康科學研究所 |
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