https://scholars.lib.ntu.edu.tw/handle/123456789/136141
標題: | 台灣杉雄配子體發育之研究(1/2) The study of male gametogenesis of Taiwania cryptomerioides Hayata |
作者: | 陳淑華 | 關鍵字: | 台灣杉;雄配子體;雄不孕;花粉發育;組織化學染色;Taiwania cryptomerioides;male gametogenesis;male sterility;pollen development;histochemical stain | 公開日期: | 2004 | 出版社: | 臺北市:國立臺灣大學生命科學系 | 摘要: | 台灣杉(Taiwania cryptomerioides Hayata ), 又名亞杉, 屬杉科 (Taxodiaceae)為台灣原產之針葉樹 種。在第一年的研究計畫中主要以組 織化學染色,研究台灣杉可孕和不孕 花粉在發育過程中各組織和細胞內的 化學變化,並探討造成花粉不孕和花 粉崩解之原因。 在發育過程中,可孕和不孕的小 孢子和營養層並無區別。於花粉成熟 時期,脂質仍能在可孕的花粉粒中發 現,但不孕者則無脂質。在四分體時 期之前,小孢子囊的花粉腔無脂質。 之後,從小孢子時期至花粉成熟時 期,不孕的花粉腔中可檢測出大量的 脂質,但可孕者則缺乏脂質。 在可孕的小孢子囊中,多醣類在 所有的發育階段皆能被測得。反之, 在小孢子時期以後,不孕者的多醣類 含量較少,且在花粉成熟時期無任何 多醣類存在。從造孢時期至二分體時 期,不論可孕或不孕的小孢子囊的營 養層,皆有多醣類存在。整體而言, 可孕和不孕的營養層的多醣類含量並 無差異。在不孕的小孢子囊中,花粉 腔含有大量的多醣類,然可孕者在所 有發育階段中,花粉腔無多醣類的出 現。 從造孢時期至小孢子母細胞晚 期,營養層中可發現大量的蛋白質。 隨後的二個時期 (即二分體時期和四 分體時期),僅有微量的蛋白質存在。 四分體時期之後,由於營養層的瓦 解,因此營養層細胞中無蛋白質的反 應。可孕和不孕小孢子囊的營養層, 其蛋白質含量無明顯的差別。從造孢 時期至小孢子母細胞晚期,小孢子含 有大量的蛋白質,爾後的時期則蛋白 質含量較少。從二分體時期至雙細胞 花粉時期,可在不孕小孢子中測得微 量的蛋白質。在花粉成熟時期,可孕 的小孢子含有豐富的蛋白質,不孕的 則無。在小孢子時期之後,不孕者的 花粉腔有蛋白質反應。反之,從小孢 子母細晚期至四分體時期,可孕者的 花粉腔有蛋白質反應,爾後的時期則 無蛋白質反應。 The endemic cypress species to Taiwan, Taiwania cryptomerioides Hayata was studies with histochemical staining in the first year. Over these developing stages, both the fertile and sterile microspore did not differ from each other in microspores and tapetum. At mature pollen grain (MPG) stage, lipids were still detectable for fertile PG, but not for sterile one. It was detectable a tremendous amount of 2 lipid granules from M to PG stages in the locule of sterile microsporangia, while only trace amount of fertile one. In the fertile microspores, polysaccharides were detectable throughout the entire developmental stages. In the sterile one, in contrast, less polysaccharide was detected after microspore stage and entirely not detected at PG stage. In the tapetum, polysaccharides were detectable from the stage of sporogenous tissue to dyad stage, either in fertile or in sterile microsporangia. As a whole, there is no significant difference between fertile and sterile microsporangia in polysaccharide content in the tapetum. In the sterile microsporangia, tremendous polysaccharides were detected in the locule, while in the fertile one none was detectable throughout the entire developmental stages. In the tapetum, a great amount of proteins was found from the stage of sporogenous tissue to that of late MMC. At subsequent two stages, namely dyad and tetrad stages, only a trace amount of proteins was detected. Later than tetrad stage, no protein was detected in the tapetum owing to the degradation of tapetum itself. In the protein content, there was not significant difference between fertile and sterile microsporangia. In microspores, there were also tremendous amount of proteins detectable from the stage of sporogenous tissue to that of late MMC, followed by less amount of protein content in the subsequent developmental stages. In the sterile microspores, less protein content was detected at the stages from dyad to BPG. At MPG stage, fertile microspores were characterized by plenty of protein contents, while sterile ones none at all. In the sterile one, proteins were detectable after microspore stage. In the fertile one, on the contrary, proteins were detected in the locule from late microspore mother cell stage to tetrad stage, but not at the subsequent stages. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/10284 | 其他識別: | 922313B002118 | Rights: | 國立臺灣大學生命科學系 |
顯示於: | 生命科學系 |
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