魚類神經壞死症病毒持續性感之研究(四)
Date Issued
2005
Date
2005
Author(s)
DOI
932311B002018
Abstract
Nervous necrosis virus (NNV),
belonging to betanodavirus of
Nodaviridae, has caused mass
mortality of many species of cultured
fish around the world. A new cell line
BB was established from the brain
tissue of a surviving barramundi
(Lates calcifer) after viral nervous
necrosis disease, and was
demonstrated to have
NNV-persistent infection. The aim of
this study was to examine if
NNV-persistent infection in the BB
cell line was related to interferon
(IFN) response. A negative control
cell line was obtained by treating
with NNV-specific rabbit antiserum
for 5 subcultures. Virus titers of the
treated BB cells drastically
decreased from 103-7 TCID50 ml-1 to
levels below 101.5 TCID50 ml-1, and
neither NNV RNA nor capsid protein
could be detected in the cured BB
(cBB) cells. The expression of Mx
3
protein, type I IFN-inducible antiviral
protein, was detected in the BB cells
but not in the cBB cells; therefore,
IFN response existed in the BB cells.
In addition, the cBB cells could also
express Mx gene through
polyinosinic-polycytidylic acid (poly
I:C) transfection and NNV infection.
The result of antiviral activity assay
indicated that IFN-like substances
existed in the supernatant of BB
cells. In conclusion, IFN protection
was responsible for the persistence
of NNV infection in BB cells.
Subjects
fish nervous necrosis virus
persistent infection
interferon
Mx gene
Publisher
臺北市：國立臺灣大學生命科學系
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
932311B002018.pdf
Size
147.66 KB
Format
Adobe PDF
Checksum
(MD5):1b1a5a4763aa20dc938a375bbbcac271