DC 欄位 | 值 | 語言 |
dc.contributor.author | 齊肖琪 | zh_TW |
dc.creator | 齊肖琪 | zh_TW |
dc.date | 2005 | zh_TW |
dc.date.accessioned | 2006-07-25T09:58:24Z | - |
dc.date.accessioned | 2018-07-06T01:22:13Z | - |
dc.date.available | 2006-07-25T09:58:24Z | - |
dc.date.available | 2018-07-06T01:22:13Z | - |
dc.date.issued | 2005 | - |
dc.identifier | 932311B002018 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/10291 | - |
dc.description.abstract | 本實驗室所建立的金目鱸腦細胞
株 (Baramundi Brain cell line, BB
cell line)是源自於爆發病毒性神經壞
死症之殘活金目鱸(Baramundi)腦組
織的細胞株,已證實有神經壞死症病毒
(Nervous Necrosis Virus, NNV)的持
續性感染。本研究在探討造成NNV 持
續性感染的機制是否與干擾素有關。
以抗NNV之多源抗體治療持續性感染
的BB細胞療後, 其上清液的病毒力價
明顯自103-7 TCID50 ml-1 下降至低於
10 1.5 TCID 50 ml-1,且完全檢測不到
NNV 病毒核酸及外鞘蛋白質的表現,
而成為不再有NNV持續性感染之負對
照組細胞 cured BB (cBB)。干擾素會
誘發細胞產生Mx protein,因此本實
驗以Mx gene 專一核酸引子檢測BB
及cBB 細胞,發現BB 細胞有Mx gene
的表現,而cBB 細胞則沒有Mx gene
的表現,因此干擾素存在於有NNV 持
續性感染的BB 細胞中。此外,以NNV
再度感染的cBB 細胞,以及以 poly
I:C 轉染(transfection) 的cBB 細胞則
有Mx gene 的表現,證明NNV 的感
染可以誘導cBB 細胞產生干擾素。在
抗病毒活性測試中,確定BB 細胞上清
液中有干擾素活性的物質,因此,干
擾素的保護效應是造成BB 細胞持續
性感染NNV 的原因。 | zh_TW |
dc.description.abstract | Nervous necrosis virus (NNV),
belonging to betanodavirus of
Nodaviridae, has caused mass
mortality of many species of cultured
fish around the world. A new cell line
BB was established from the brain
tissue of a surviving barramundi
(Lates calcifer) after viral nervous
necrosis disease, and was
demonstrated to have
NNV-persistent infection. The aim of
this study was to examine if
NNV-persistent infection in the BB
cell line was related to interferon
(IFN) response. A negative control
cell line was obtained by treating
with NNV-specific rabbit antiserum
for 5 subcultures. Virus titers of the
treated BB cells drastically
decreased from 103-7 TCID50 ml-1 to
levels below 101.5 TCID50 ml-1, and
neither NNV RNA nor capsid protein
could be detected in the cured BB
(cBB) cells. The expression of Mx
3
protein, type I IFN-inducible antiviral
protein, was detected in the BB cells
but not in the cBB cells; therefore,
IFN response existed in the BB cells.
In addition, the cBB cells could also
express Mx gene through
polyinosinic-polycytidylic acid (poly
I:C) transfection and NNV infection.
The result of antiviral activity assay
indicated that IFN-like substances
existed in the supernatant of BB
cells. In conclusion, IFN protection
was responsible for the persistence
of NNV infection in BB cells. | en |
dc.format | application/pdf | zh_TW |
dc.format.extent | 151205 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學生命科學系 | zh_TW |
dc.rights | 國立臺灣大學生命科學系 | zh_TW |
dc.subject | 魚類神經壞死症病毒 | zh_tw |
dc.subject | 持續性感染 | zh_tw |
dc.subject | 干擾素 | zh_tw |
dc.subject | Mx 基因 | zh_tw |
dc.subject | fish nervous necrosis virus | en |
dc.subject | persistent infection | en |
dc.subject | interferon | en |
dc.subject | Mx gene | en |
dc.title | 魚類神經壞死症病毒持續性感之研究(四) | zh_TW |
dc.type | report | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/10291/1/932311B002018.pdf | - |
dc.coverage | 計畫年度:93;起迄日期:2004-08-01/2005-07-31 | zh_TW |
item.openairecristype | http://purl.org/coar/resource_type/c_93fc | - |
item.openairetype | report | - |
item.languageiso639-1 | zh_TW | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
crisitem.author.orcid | 0000-0003-1738-6205 | - |
顯示於: | 生命科學系
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