The Adhesion of Abalone (Haliotis Diversicolor) Hemocyte is Regulated by Protein Kinase A Signal Transduction Pathway

The adhesion of hemocytes plays an important role in the innate immunity of abalones. According to the results from this study, several conclusions about the role of PKA (protein kinase A) in the adhesion of abalone hemocytes. First, during cell adhesion, the amount of cAMP decreased in hemocytes. Second, elevation of cAMP inhibited cell adhesion. Third, the PKA inhibitor KT5720 enhanced the cell adhesion. Fourth, PKA activity decreased following the time course of incubation. Totally, these results strongly inferred that the adhesion of abalone hemocyte is regulated by PKA and the related signal transduction pathway. Horever, the another cAMP-mediated protein Epac wasn’t involved to regulate the adhesion of hemocytes. Besides, due to RGD peptide could affect the cell adhesion, it suggested that the adhesion of abalone hemocytes was mediated by the interaction between integrin & fibronection.