Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Life Science / 生命科學院
  3. Life Science / 生命科學系
  4. Comparison of Transmission at Synapses of Layers 2/3 Input onto Layer 5 Pyramidal and GABAergic Neurons in Rostral Agranular Insular Cortex
 
  • Details

Comparison of Transmission at Synapses of Layers 2/3 Input onto Layer 5 Pyramidal and GABAergic Neurons in Rostral Agranular Insular Cortex

Date Issued
2016
Date
2016
Author(s)
Chang, Ting-Hsuan Daniel
DOI
10.6342/NTU201601485
URI
http://ntur.lib.ntu.edu.tw//handle/246246/272388
Abstract
It has been shown that inhibition or lesion of the rostral agranular insular cortex (RAIC) results in analgesia, it suggests that RAIC tonically produces hyperalgesia signal. RAIC is a cortical area where nociceptive output originates, and it has been reported to activate in chronic pain perception. It’s believed that chronic pain is associated with the long-term change in synaptic plasticity. Moreover, the imbalance of excitatory and inhibitory (E/I) synaptic signaling in neural circuits is responsible to modulate synaptic plasticity in certain behavior disorders. In our lab, previous study had reported that the induction of chronic pain induced differential activation in pyramidal cells and GABAergic neurons in RAIC. We propose here that E/I imbalance in RAIC may contribute to the increased cortical output of nociceptive signal in chronic pain. To test this possibility, we compared synaptic transmission of layers 2/3 (L2/3) inputs onto layer 5 (L5) pyramidal cells (PC), which are the descending projection neurons, and onto local GABAergic interneurons (IntN) in RAIC. We performed dual-patch recording from a paired IntN-PC in layer 5, and elicited EPSC by putting an electrode in layer 2/3. We found functional connectivity in 34.2% of all recorded IntN-PC pairs. There was no significant difference in data sampled from IntN-PC pairs with and without functional connectivity, and all data were pooled. Our data showed no significant difference in paring-pulse ratio between transmission at L2/3-PC synapses and at L2/3-IntN synapses. L2/3-IntN seemed to have higher releasing probability than L2/3-PC synapse in quantum study. The ratio of NMDA and non-NMDA EPSCs component was larger at L2/3-PC synapses than at L2/3-IntN synapses. Furthermore, the rising and decay of EPSCs were much faster at L2/3-IntN synapse than at L2/3-PC synapse. We further examined the modulation of pERK on IntN-PC pairs by applying PKC activator Phorbol 12,13- diacetate (PDA). PDA enhanced the postsynaptic currents at L2/3-PC synapses and L2/3- IntN synapses. The further issue of chronic pain model is under studying.
Subjects
rostral agranular insular cortex (RAIC)
E-I ratio
pain
pERK
whole-cell patch clamp
Type
thesis
File(s)
Loading...
Thumbnail Image
Name

ntu-105-R02b21009-1.pdf

Size

23.32 KB

Format

Adobe PDF

Checksum

(MD5):16c33be6215048967550407cd4c5aec3

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science