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  4. Expression analysis of stathmin family genes in zebrafish embryos
 
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Expression analysis of stathmin family genes in zebrafish embryos

Date Issued
2016
Date
2016
Author(s)
Lin, Shuo-Peng
DOI
10.6342/NTU201600314
URI
http://ntur.lib.ntu.edu.tw//handle/246246/272395
Abstract
Microtubules are well known to mediate many physiological processes in eukaryotic cell such as stable cell structure and axonal outgrowth. Microtubules can be regulated by different microtubule binding proteins to mediated their assembly and disassembly. Stathmin family proteins are phosphoprotein that regulates microtubules disassembly. Stathmin interacts with microtubules by binding to two moles of alpha and beta tubulin dimer to form a tetramer. Stathmins also interfere with microtubule polymerization by direct binding to microtubules. Via tight regulation of microtubule polymerization and depolymerization, stathmins are involved in different cellular processes, including neurogenesis, cell proliferation, differentiation and activities. In mammals, stmn 1 is ubiquitous expressed while other stathmin members, stmn 2, stmn 3, stmn 4 are mainly expressed in the nervous system. Previously, we have demonstrated that stmn 2, one of the zebrafish stathmin proteins, plays a vital role in zebrafish brain development. There are seven different stathmin genes in zebrafish. To understand the role of stathmin in zebrafish, I have cloned stmn 1a, stmn 2b and stmn 4l and demonstrated that they shared high identity with other vertebrate stathmin homologues. I analyzsed their temporal expression patterns using embryos at different stages by whole-mount in situ hybridization. I observed that stmn 1a and stmn 2b were expressed in spinal cord and trigeminal ganglia from 16 h post fertilization (hpf) but stmn 4l appeared from 18 hpf. stmn2b and stmn4l were mainly expressed in the central nervous system (CNS). Furthermore, the stmn1a transcripts were found in eyes, pharyngeal arches and tail bud. stmn 1a, stmn 2b and stmn4l were all expressed in hypothalamus, tegmentum, telencephalon and diencephalon after 24 hpf. After 48 hpf, stmn2b and stmn4l were still expressed in the CNS while stmn1a did not expressed in hindbrain. The dynamic expression of stathmin family genes in the developing central nervous system suggests a pivotal roles of those genes in regulating CNS development in zebrafish.
Subjects
zebrafish
stathmmin
embryo
Type
thesis
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