β-Amylase is not Involved in Degradation of Endosperm Starch During Seed Germination of Maize
Resource
Taiwania,49(四)
Journal
Taiwania
Journal Volume
49
Journal Issue
四
Pages
-
Date Issued
2004
Date
2004
Author(s)
Wang, Shue-Mei
Yang, Fu-Mee
Chou, Ai-Hsiang
DOI
246246/2006121215550983
Abstract
For species of Triticeae, e.g., barley, β-amylase was synthesized in endosperm during
seed development and processed to active form during seed germination. Thus, β-amylase was
suggested to be one of the enzymes to degrade endosperm starch for seed germination. β-Amylase
expression in maize kernels apparently is different from that of barley. By immunolocalization, we
found that maize β-amylase is expressed in the pericarp but not in the endosperm during seed
development; and is synthesized in the aleurone cells but not in the scutellar epithelium cells during
seed germination. The newly synthesized maize β-amylase is retained inside of the aleurone cells. On
the contrary, maize α-amylase is synthesized and secreted from the aleurone cells to the starchy
endosperm. By PAS and iodine stain, we showed that starch is present in the starchy endosperm and
absent in the aleurone cells of maize seeds. Results of starch film assay and native activity gel analysis
indicated that α-amylases are the enzymes for endosperm starch degradation during maize seed
germination. Because maize β-amylase is synthesized and retained in the aleurone cells, it is unlikely
that β-amylase acts on endosperm starch during seed germination.
Subjects
Maize
β-amylase
Immunolocalization
Publisher
臺北市：國立台灣大學生態學與演化生物學研究所
Type
other
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