https://scholars.lib.ntu.edu.tw/handle/123456789/136564
標題: | Functional consequences of naturally occurring DRY motif variants in the mammalian chemoattractant receptor GPR33 | 作者: | Römpler Holger Yu, Hon-Tsen Arnold, Antje Orth, Annie Schöneberg, Torsten |
關鍵字: | Chemokine; Constitutive activity; DRY motif; Evolution; G-protein-coupled receptor; Pseudogene | 公開日期: | 2006 | 卷: | 87 | 期: | 6 | 起(迄)頁: | 724-732 | 來源出版物: | Genomics | 摘要: | Most members of the large family of rhodopsin-like G-protein-coupled receptors possess an evolutionarily conserved Asp-Arg-Tyr (DRY) motif in the C-terminal region of the third transmembrane domain. Mutations of residues within this motif usually abolish receptor function and, when they occur naturally, can even cause human diseases. By analyzing over 100 mammalian orthologs of the chemoattractant receptor GPR33 we identified several polymorphic and fixed sequence variations within the DRY motif. Unexpectedly, the naturally occurring mutation of Arg3.50 to His in mouse GPR33 showed no difference from the wild-type receptor in several functional tests. Sequence analysis of GPR33 from Asian house mice revealed the polymorphic existence of Arg3.50 and His3.50 alleles in wild-trapped populations, further supporting the functional equivalence of both allelic variants. In contrast, the Arg3.50 to Gly mutation found in hamster GPR33 inactivates the receptor and may have contributed to pseudogenization of this gene in this species. Functional data with GPR33 variants indicate different receptor- and context-specific consequences of DRY mutations. Our study also reveals GPR33 as a new example illustrating missense mutations as a first step in the pseudogenization process. ? 2006 Elsevier Inc. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/161628 https://www.scopus.com/inward/record.uri?eid=2-s2.0-33646936560&doi=10.1016%2fj.ygeno.2006.02.009&partnerID=40&md5=f0c617c79f36c98561e3800a69ab8ae8 |
ISSN: | 08887543 | DOI: | 10.1016/j.ygeno.2006.02.009 | SDG/關鍵字: | arginine; aspargylarginyltyrosine; chemoattractant; G protein coupled receptor; glycine; histidine; protein gpr33; tyrosine derivative; unclassified drug; allele; article; controlled study; function test; gene function; gene mutation; gene sequence; genetic polymorphism; genetic variability; hamster; in vitro study; mammal; mouse; nonhuman; nucleotide sequence; orthology; priority journal; protein motif; pseudogene; sequence analysis; wild type; Amino Acid Motifs; Amino Acid Sequence; Amino Acid Substitution; Animals; Animals, Wild; Cell Line; Cercopithecus aethiops; COS Cells; Cricetinae; Humans; Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; Point Mutation; Pseudogenes; Receptors, G-Protein-Coupled; Sequence Homology, Amino Acid; Variation (Genetics); Cricetinae; Mammalia; Mus musculus |
顯示於: | 生命科學系 |
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