https://scholars.lib.ntu.edu.tw/handle/123456789/136929
標題: | White spot syndrome virus protein ICP11: A histone-binding DNA mimic that disrupts nucleosome assembly | 作者: | Wang, Hao-Ching Wang, Han-Ching Ko, Tzu-Ping Lee, Yu-May Leu, Jiann-Horng Ho, Chun-Han Huang, Wei-Pang Lo, Chu-Fang Wang, Andrew H.-J. |
關鍵字: | Apoptosis; Crystal structure; DNase enhancer; Shrimp aquaculture | 公開日期: | 2008 | 卷: | 105 | 期: | 52 | 起(迄)頁: | 20758-20763 | 來源出版物: | Proceedings of the National Academy of Sciences | 摘要: | White spot syndrome virus (WSSV) is a large (?300 kbp), double-stranded DNA eukaryotic virus that has caused serious disease in crustaceans worldwide. ICP11 is the most highly expressed WSSV nonstructural gene/protein, which strongly suggests its importance in WSSV infection; but until now, its function has remained obscure. We show here that ICP11 acts as a DNA mimic. In crystal, ICP11 formed a polymer of dimers with 2 rows of negatively charged spots that approximated the duplex arrangement of the phosphate groups in DNA. Functionally, ICP11 prevented DNA from binding to histone proteins H2A, H2B, H3, and H2A.x, and in hemocytes from WSSV-infected shrimp, ICP11 colocalized with histone H3 and activated-H2A.x. These observations together suggest that ICP11 might interfere with nucleosome assembly and prevent H2A.x from fulfilling its critical function of repairing DNA double strand breaks. Therefore, ICP11 possesses a functionality that is unique among the handful of presently known DNA mimic proteins. ? 2008 by The National Academy of Sciences of the USA. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/162050 http://ntur.lib.ntu.edu.tw/bitstream/246246/162050/1/06.pdf |
DOI: | 10.1073/pnas.0811233106 | SDG/關鍵字: | amino acid derivative; deoxyribonuclease; double stranded DNA; histone H2A; histone H2B; histone H3; polymer; protein icp11; unclassified drug; virus DNA; virus protein; article; cell structure; controlled study; DNA binding; human; human cell; immunofluorescence test; in vivo study; nucleosome; priority journal; protein function; protein localization; White spot syndrome virus; Animals; Crystallography, X-Ray; Dimerization; DNA; Hemocytes; Histones; Nucleosomes; Penaeidae; Protein Binding; Protein Structure, Quaternary; Viral Proteins; White spot syndrome virus 1; Crustacea; Decapoda (Crustacea); Eukaryota; Shrimp white spot syndrome virus |
顯示於: | 生命科學系 |
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