https://scholars.lib.ntu.edu.tw/handle/123456789/136938
標題: | In Vitro Differentiation of Size-Sieved Stem Cells into Electrically Active Neural Cells | 作者: | Hung, Shih-Chieh Cheng, Henrich Pan, Chien-Yuan Tsai, May J. Kao, Lung-Sen Ma, Hsiao-Li |
關鍵字: | Bone marrow stromal cells; Mesenchymal stem cells; Neural differentiation; Plastic-adherent cells | 公開日期: | 2002 | 卷: | 20 | 期: | 6 | 起(迄)頁: | 522-529 | 來源出版物: | Stem Cells | 摘要: | Size-sieved stem (SS) cells isolated from human bone marrow and propagated in vitro are a population of cells with consistent marker typing, and can form bone, fat, and cartilage. In this experiment, we demonstrated that SS cells could be induced to differentiate into neural cells under experimental cell culture conditions. Five hours after exposure to antioxidant agents (β-mercaptoethanol ± retinoic acid) in serum-free conditions, SS cells expressed the protein for nestin, neuron-specific enolase (NSE), neuron-specific nuclear protein (NeuN), and neuron-specific tubulin-1 (TuJ-1), and the mRNA for NSE and Tau. Immunofluoreseence showed that almost all the cells (>98%) expressed NeuN and TuJ-1. After 5 days of β-mercaptoethanol treatment, the SS cells expressed neurofilament high protein but not mitogen-activated protein-2, glial filament acidic protein, and galactocerebroside. For such long-term-treated cells, voltage-sensitive ionic current could be detected by electrophysiological recording, and the intracellular calcium ion, Ca2+, concentration can be elevated by high potassium (K+) buffer and glutamate. These findings suggest that SS cells may be an alternative source of undifferentiated cells for cell therapy and gene therapy in neural dysfunction. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/162059 http://ntur.lib.ntu.edu.tw/bitstream/246246/162059/1/02.pdf |
DOI: | 10.1634/stemcells.20-6-522 | SDG/關鍵字: | galactosylceramide; glial fibrillary acidic protein; ion channel; mercaptoethanol; nestin; neuron specific enolase; neuron specific nuclear protein; neuron specific tubulin 1; retinoic acid; tau protein; unclassified drug; antineoplastic agent; calcium; mercaptoethanol; nerve protein; retinoic acid; adoptive immunotherapy; article; bone marrow; calcium cell level; cell culture; cell differentiation; cell population; controlled study; culture medium; electrophysiology; electrostimulation; gene therapy; human; human cell; immunofluorescence; ion current; nerve cell; neurofilament; neurologic disease; protein expression; stem cell; cell differentiation; cell membrane potential; cell size; culture medium; culture technique; cytology; drug effect; fluorescent antibody technique; genetics; hematopoietic stem cell; in vitro study; metabolism; methodology; patch clamp; physiology; Western blotting; Antineoplastic Agents; Blotting, Western; Calcium; Cell Culture Techniques; Cell Differentiation; Cell Size; Culture Media; Fluorescent Antibody Technique; Hematopoietic Stem Cells; Humans; Membrane Potentials; Mercaptoethanol; Nerve Tissue Proteins; Neurons; Patch-Clamp Techniques; Tretinoin |
顯示於: | 生命科學系 |
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