https://scholars.lib.ntu.edu.tw/handle/123456789/136948
標題: | Calcium influxes and mitogen-activated protein kinase kinase activation mediate ethylene inducing ipomoelin gene expression in sweet potato | 作者: | CHEN, YU-CHI LIN, HSIN-HUNG JENG, SHIH-TONG |
關鍵字: | Calcium ion; Ethylene signalling; Membrane channel | 公開日期: | 2008 | 起(迄)頁: | 071030013314001-??? | 來源出版物: | Plant Cell & Environment | 摘要: | The ipomoelin gene (IPO) was identified to be a wound-inducible gene from Ipomoea batatas, and its expression was stimulated by methyl jasmonate (MeJA) and hydrogen peroxide. IPO protein was also characterized as a defence-related protein, and it is also a carbohydrate-binding protein. In this study, the expression of IPO was used as a molecular probe to study the effects of Ca 2+ on the signal transduction of ethylene. A confocal microscope monitored the Ca2+ within cells, and Northern blotting examined IPO expression. The presence of Ca2+ channel blocker, including diltiazem, neomycin or ruthenium red, abolished the increase of cytosolic Ca2+, and reduced the IPO expression in the cells induced by ethylene. Furthermore, both Ca2+ influxes and IPO expression stimulated by ethylene were prohibited in the presence of 10 mm ethylene glycol-bis(2-aminoethyl ether)-N, N, N′, N′-tetraacetic acid (EGTA). These results indicated that Ca2+ influxes into the cytosol induced by ethylene are from both apoplast and organelles, and are required for activating IPO expression. However, in the presence of 1 mm EGTA, ethylene can still stimulate IPO expression, but mechanical wounding failed to do it. Therefore, Ca2+ channels in the plasma membrane induced by ethylene have higher affinity to Ca2+ than that stimulated by wounding. Moreover, the addition of A23187, an ionophore, raised cytosolic Ca 2+, but was unable to stimulate IPO expression. These findings showed that IPO induction did not solely depend on Ca2+, and Ca 2+ elevation in cytosol is necessary but not sufficient for IPO expression. The application of PD98059, a mitogen-activated protein kinase kinase (MAPKK) inhibitor, did not prevent Ca2+ from increasing in the cytosol induced by ethylene, but inhibited the IPO expression stimulated by staurosporine (STA), a protein kinase inhibitor. Conclusively, elevation of cytosolic Ca2+ by ethylene may stimulate protein phosphatase and MAPKK, which finally activates IPO expression. © 2007 The Authors. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/162069 http://ntur.lib.ntu.edu.tw/bitstream/246246/162069/1/13.pdf https://www.scopus.com/inward/record.uri?eid=2-s2.0-36749039809&doi=10.1111%2fj.1365-3040.2007.01742.x&partnerID=40&md5=cdeb055da44a76cf8acac2913e5f8848 |
ISSN: | 01407791 | DOI: | 10.1111/j.1365-3040.2007.01742.x | SDG/關鍵字: | 2 (2 amino 3 methoxyphenyl)chromone; calcium; egtazic acid; ethylene; ethylene derivative; flavonoid; ipomoelin protein, Ipomoea batatas; mitogen activated protein kinase kinase; unclassified drug; vegetable protein; antibiotics; calcium; carbohydrate; cation; enzyme activity; ethylene; gene expression; hydrogen peroxide; inhibitor; protein; tuber; vine; wounding; article; drug effect; enzyme activation; enzymology; gene expression regulation; genetics; metabolism; signal transduction; sweet potato; Calcium; Egtazic Acid; Enzyme Activation; Ethylenes; Flavonoids; Gene Expression Regulation, Plant; Ipomoea batatas; Mitogen-Activated Protein Kinase Kinases; Plant Proteins; Signal Transduction; Ipomoea batatas |
顯示於: | 生命科學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。