https://scholars.lib.ntu.edu.tw/handle/123456789/138460
標題: | Bitter melon (Momordica charantia L.) inhibits adipocyte hypertrophy and down regulates lipogenic gene expression in adipose tissue of diet-induced obese rats | 作者: | Huang, Hui-Ling Hong, Ya-Wen Wong, You-Hong Chen, Ying-Nien Chyuan, Jong-Ho Huang, Ching-Jang Chao, Pei-Min |
關鍵字: | Adipocyte hypertrophy; Bitter melon; Lipogenic genes; Peroxisome proliferator-activated receptor; Thiazolidinedione | 公開日期: | 2008 | 來源出版物: | British Journal Of Nutrition | 摘要: | Bitter melon (Momordica charantia; BM) has been shown to ameliorate diet-induced obesity and insulin resistance. To examine the effect of BM supplementation on cell size and lipid metabolism in adipose tissues, three groups of rats were respectively fed a high-fat diet supplemented without (HF group) or with 5% lyophilised BM powder (HFB group), or with 0.01% thiazolidinedione (TZD) (HFT group). A group of rats fed a low-fat diet was also included as a normal control. Hyperinsulinaemia and glucose intolerance were observed in the HF group but not in HFT and HFB groups. Although the number of large adipocytes (>180 μm) of both the HFB and HFT groups was significantly lower than that of the HF group, the adipose tissue mass, TAG content and glycerol-3-phosphate dehydrogenase activity of the HFB group were significantly lower than those of the HFT group, implying that BM might reduce lipogenesis in adipose tissue. Experiment 2 was then conducted to examine the expression of lipogenic genes in adipose tissues of rats fed low-fat, HF or HFB diets. The HFB group showed significantly lower mRNA levels of fatty acid synthase, acetyl-CoA carboxylase-1, lipoprotein lipase and adipocyte fatty acid-binding protein than the HF group (P < 0.05). These results indicate BM can reduce insulin resistance as effective as the anti-diabetic drug TZD. Furthermore, BM can suppress the visceral fat accumulation and inhibit adipocyte hypertrophy, which may be associated with markedly down regulated expressions of lipogenic genes in the adipose. ? 2007 The Authors. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/162623 | DOI: | 10.1017/S0007114507793947 | SDG/關鍵字: | acetyl coenzyme A carboxylase; fatty acid binding protein; fatty acid synthase; glucose; glycerol 3 phosphate dehydrogenase (NAD); insulin; lipoprotein lipase; Momordica charantia extract; pioglitazone; triacylglycerol; adipose tissue cell; animal experiment; animal model; animal tissue; article; cell size; controlled study; down regulation; gene expression; glucose blood level; glucose intolerance; hyperinsulinemia; insulin blood level; insulin resistance; lipid metabolism; lipogenesis; lipohypertrophy; nonhuman; nucleotide sequence; obesity; oral glucose tolerance test; rat; RNA isolation; triacylglycerol blood level; weight reduction; Adipocytes; Adipose Tissue; Animals; Anti-Obesity Agents; Cell Size; Diet; Dietary Fats; Dietary Supplements; Down-Regulation; Eating; Glucose Tolerance Test; Hypertrophy; Insulin Resistance; Lipid Metabolism; Lipogenesis; Male; Momordica charantia; Obesity; Organ Size; Phytotherapy; Plant Extracts; Rats; Rats, Wistar; Weight Gain; Cucumis; Momordica charantia; Rattus |
顯示於: | 生化科技學系 |
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