https://scholars.lib.ntu.edu.tw/handle/123456789/138636
標題: | Proteomic Analysis of Caco-2 Cells Treated with Monacolin K | 作者: | Lin, Wun-Yuan Song, Ching-Yung Pan, Tzu-Ming |
關鍵字: | Anti-cancer; Monacolin K; Monascus; Proteomics | 公開日期: | 2006 | 卷: | 54 | 期: | 17 | 起(迄)頁: | 6192-6200 | 來源出版物: | Journal of Agricultural and Food Chemistry | 摘要: | Monascus species is an important traditional fermentation fungus used on food. Monacolin K (a secondary metabolite of Monascus, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase) in inhibition of mevalonate synthesis may result in reductions of isoprenoid prenylation in cells. Impairment of protein isoprenoid prenylation has been related to anticancer effect in cancer cells. As a functional food for Monascus, however, the molecular mechanisms responsible for the anti-proliferate effect of monacolin K in cancer cells are not clear. We used proteomic analysis by two-dimensional gel electrophoresis, matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS), tandem mass spectrometry (MS/MS), and database interrogation to separate and identify the proteins of Caco-2 cells treated with monacolin K. The results showed that monacolin K inhibited the proliferation of Caco-2 cells in a dose-dependent manner. The identified proteins in proteomic analysis included anti-oxidation enzymes related to reactive oxygen species stress, cytoskeleton proteins, glycolytic enzymes, and enzymes involved in mediating protein interactions. Furthermore, glutathione S-transferase P 1 and cytoskeleton-8, -18, and -19 revealed a down-regulation in a dose-dependent manner in exposure of Caco-2 cells to monacolin K. ? 2006 American Chemical Society. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/162700 http://ntur.lib.ntu.edu.tw/bitstream/246246/162700/1/46.pdf |
DOI: | 10.1021/jf061060c | SDG/關鍵字: | antineoplastic agent; mevinolin; tumor protein; article; cell division; cell strain CACO 2; cell survival; chemistry; colon tumor; dose response; drug effect; gene expression; genetics; human; mass spectrometry; pathology; protein processing; proteomics; two dimensional gel electrophoresis; Western blotting; Antineoplastic Agents; Blotting, Western; Caco-2 Cells; Cell Division; Cell Survival; Colonic Neoplasms; Dose-Response Relationship, Drug; Electrophoresis, Gel, Two-Dimensional; Gene Expression; Humans; Lovastatin; Neoplasm Proteins; Protein Isoprenylation; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Fungi; Monascus |
顯示於: | 生化科技學系 |
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