https://scholars.lib.ntu.edu.tw/handle/123456789/139254
標題: | Analysis of the cellulose synthase genes associated with primary cell wall synthesis in Bambusa oldhamii | 作者: | Chen, Chih-Yu Hsieh, Meng-Hsun Yang, Chien-Chih Lin, Choun-Sea Wang, Ai-Yu |
關鍵字: | Bamboo; Bambusa oldhamii; cDNA cloning; Cellulose synthase; In situ RT-PCR; Poaceae; Primary cell wall; Quantitative real-time PCR | 公開日期: | 2010 | 卷: | 71 | 期: | 11-12 44877 |
起(迄)頁: | 1270-1279 | 來源出版物: | Phytochemistry | 摘要: | The synthesis of cell wall polysaccharides is highly active in rapidly growing bamboo shoots. We cloned a set of BoCesA cDNAs that encode cellulose synthase from bamboo (Bambusa oldhamii) and investigated the expression patterns of the BoCesA2, BoCesA5, BoCesA6 and BoCesA7 genes. The four BoCesA genes were differentially expressed in the different parts of growing bamboo shoots, in various organs, and in multiple shoots that were cultured in vitro. They were down-regulated by α-naphthaleneacetic acid and differentially affected by thidiazuron in the multiple shoots. In situ RT-PCR analyses demonstrated that BoCesA2, BoCesA5, BoCesA6, and BoCesA7 mRNAs were present throughout the base and the internode regions of the etiolated shoots that emerged from pseudorhizomes, and in the internode regions of the juvenile branch shoots that emerged from nodes of mature bamboo culms; however, the expression of the four genes in the lignified internode of the branch shoot was predominantly detected in the center of the vascular bundles. Our results for cDNA cloning, expression analyses, and phylogenetic analysis suggest that the 10 BoCesA genes cloned from the etiolated bamboo shoots participate in cellulose synthesis in the primary cell walls of the growing bamboo, and that at least three additional BoCesA genes involved in cellulose synthesis in the secondary walls may be present in the bamboo genome. The expressions of BoCesA genes may be under fine control in response to the various developmental stages and physiological conditions of bamboo. © 2010 Elsevier Ltd. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/243183 http://ntur.lib.ntu.edu.tw/bitstream/246246/243183/-1/125.pdf https://www.scopus.com/inward/record.uri?eid=2-s2.0-77954242016&doi=10.1016%2fj.phytochem.2010.05.011&partnerID=40&md5=ae4f59a524a4261a0aea702b630196f5 |
ISSN: | 00319422 | DOI: | 10.1016/j.phytochem.2010.05.011 | SDG/關鍵字: | 1 naphthylacetic acid; 1-naphthaleneacetic acid; arylacetic acid derivative; cellulose synthase; glucosyltransferase; glucosyltransferase; article; Bambusa; cell wall; chemistry; genetics; growth, development and aging; metabolism; plant leaf; reverse transcription polymerase chain reaction; metabolism; Bambusa; Cell Wall; Glucosyltransferases; Naphthaleneacetic Acids; Plant Leaves; Reverse Transcriptase Polymerase Chain Reaction; Bambusa; Dendrocalamopsis oldhamii; Poaceae; Bambusa; Cell Wall; Glucosyltransferases; Naphthaleneacetic Acids; Plant Leaves; Reverse Transcriptase Polymerase Chain Reaction |
顯示於: | 生化科技學系 |
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