https://scholars.lib.ntu.edu.tw/handle/123456789/139493
標題: | 阿拉伯芥中FIN219基因的功能性研究(1/3) | 作者: | 謝旭亮 | 關鍵字: | FIN219;FIP1(FIN219-interacting protein 1);GST(glutathione-S-transferase);光敏素A;B;茉莉酸(jasmonate);Phytochrome A;Jasmonate | 公開日期: | 31-七月-2004 | 出版社: | 臺北市:國立臺灣大學植物科學研究所 | 摘要: | FIN219 已被證實參與阿拉伯芥中光敏素A 的訊息傳遞,並且是COP1 的一個基 因外抑制體。為了進一步的瞭解FIN219 在植物發育過程與光訊息傳導過程中所 扮演的角色,我們利用yeast two-hybrid 的方法篩選可與FIN219 相互作用的蛋 白質。一個產生GST ( glutathione-S-transferase ) 蛋白的基因FIP1 (FIN219-interacting protein 1)已被分離,並且它所產生的蛋白質被證實可與 FIN219 的C 端作用。大量表現FIP1 基因於Columbia 野生型時,它的轉殖株表 現出對遠紅光、紅光不敏感的長下胚軸外表型,而此外表型受FIN219 的調控; 但抑制FIP1 表現的轉殖株則表現出相反的外表型,此結果暗示FIP1 很可能是一 個負調控者,參與遠紅光對下胚軸延長的抑制性。另外,Northern blot 分析的結 果顯示FIP1 基因可受白光的誘導,並且表現於遠紅光與藍光,但在黑暗與紅光 中未檢測出它的表現。除此之外,它的mRNA 表現在遠紅光下,在光敏素A 與 fin219、fhy3 、cop1 與hy5 突變體中亦降低;而且在紅光下也受phyB 與fhy1 突 變體的影響而降低。而FIP1 的mRNA 的表現亦受auxin、jasmonate 與殺草藥的 保護劑的誘導。利用洋蔥表皮細胞的transient assay 中發現GUS 與FIP1 的轉譯 融合蛋白同時位於細胞核與細胞質中,且光並不會改變它在細胞內的位置。綜合 以上的結果顯示FIP1 是一個可與FIN219 作用,且參與光訊息傳遞,特別是遠紅 光、紅光,與多個荷爾蒙訊息傳遞的溝通者。 FIN219 has been shown to be involved in phytochrome A-mediated signaling pathway and acts as a suppressor of COP1 in Arabidopsis. To further understand the function of FIN219 in light signaling during Arabidopsis development, a yeast two-hybrid approach was utilized to isolate FIN219-interacting partners. A gene FIP1 (FIN219-interacting protein1) encoding a GST(glutathione S-transferase) was recovered and demonstrated to interact with the C-terminus of FIN219. The transgenic plants overexpressing the FIP1 gene in wild type Columbia display a hyposensitive phenotype with longer hypocotyls under continuous far-red and red light and this longer hypocotyl phenotype is dependent on the presence of the functional FIN219; however, FIP1 antisense lines show an opposite phenotype, suggesting that FIP1 may act as a negative regulator for FR-mediated inhibition of hypocotyl elongation. Furthermore, the results of Northern blot analyses indicated that FIP1 gene expression is upregulated by white light and detectable in FR as well as B light, but its expression is hardly detected in both dark and red light condition. Besides, its expression is reduced in phytochrome A mutants and several light signaling transducer such as fin219, fhy3, cop1 and hy5 mutants in continuous far red light condition and also decreased in phyB and fhy1 mutants in continuous R light. Furthermore, FIP1 expression is also induced by auxin, jasmonate and herbicide safeners. The GUS-FIP1 fusion protein is localized in both nucleus and cytoplasm based on the result of transient assays in onion cells. Taking all current results together, FIP1 is interacting with FIN219 and acts as a crosstalk between light signaling and phytohormone signaling. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/17462 | 其他識別: | 922311B002027 | Rights: | 國立臺灣大學植物科學研究所 |
顯示於: | 植物科學研究所 |
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