https://scholars.lib.ntu.edu.tw/handle/123456789/139588
標題: | DNA vaccination against foot-and-mouth disease via electroporation: study of molecular approaches for enhancing VP1 antigenicity | 作者: | Kim, Suk-Am Liang, Chi-Ming Cheng, Ivan-Chen Cheng, Yung-Chih Chiao, Ming-Tsang Tseng, Chia-Jen Lee, Fan Jong, Ming-Hwa Tao, Mi-Hua Yang, Ning-Sun Liang, Shu-Mei |
關鍵字: | Capsid protein; DNA vaccination; Electroporation immunogenicity; FMDV | 公開日期: | 2006 | 卷: | 8 | 期: | 9 | 起(迄)頁: | 1182-1191 | 來源出版物: | The Journal of Gene Medicine | 摘要: | Background: Foot-and-mouth disease virus (FMDV) affects susceptible livestock animals and causes disastrous economic impact. Immunization with plasmid expressing VP1 that contains the major antigenic epitope(s) of FMDV as cytoplasmic protein (cVP1) failed to elicit full protection against FMDV challenge. Materials and methods: In this study, mice were immunized via electroporation with four cDNA expression vectors that were constructed to express VP1 of FMDV, as cytoplasmic (cVP1), secreted (sVP1), membrane-anchored (mVP1) or capsid precursor protein (P1), respectively, to evaluate whether expression of VP1 in specific subcellular compartment(s) would result in better immune responses. Results: Electroporation enhanced immune responses to vectors expressing cVP1 or P1 and expedited the immune responses to vectors expressing sVP1 or mVP1. Immunization of mice via electroporation with mVP1 cDNA was better than sVP1 or cVP1 cDNA in eliciting neutralizing antibodies and viral clearance protection. Vaccination with P1 cDNA, nonetheless, yielded the best immune responses and protection among all four cDNAs that we tested. Conclusions: These results suggest that the antigenicity of a VP1 DNA vaccine can be significantly enhanced by altering the cellular localization of the VP1 antigen. Electroporation is a useful tool for enhancing the immune responses of vectors expressing VP1 or P1. By mimicking FMDV more closely than that of transgenic VP1 and eliciting immune responses favorably toward Th2, transgenic P1 may induce more neutralizing antibodies and better protection against FMDV challenge. Copyright ? 2006 John Wiley & Sons, Ltd. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/162823 | ISSN: | 1099498X | DOI: | 10.1002/jgm.941 | SDG/關鍵字: | capsid precursor potein; capsid protein; complementary DNA; DNA vaccine; foot and mouth disease vaccine; neutralizing antibody; protein VP1; unclassified drug; animal cell; animal experiment; animal model; antibody production; article; clinical effectiveness; controlled study; drug antigenicity; electroporation; expression vector; female; foot and mouth disease; gene construct; gene delivery system; gene therapy; hamster; immune response; immunization; molecular genetics; mouse; nonhuman; priority journal; protein localization; Th2 cell; vaccination; Animalia; Foot-and-mouth disease virus; Miridae |
顯示於: | 植物科學研究所 |
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2006_J Gene Med_DNA vaccination against FMD via electroporation_enhancing VP1 antigenicity .doc | 614.93 kB | Microsoft Word | 檢視/開啟 |
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