Tomato RAV Transcription Factor Is a Pivotal Modulator Involved in the AP2/EREBP-Mediated Defense Pathway
Resource
PLANT PHYSIOLOGY, 156(1), 213-227
Journal
Plant Physiology
Journal Volume
156
Journal Issue
1
Pages
213-227
Date Issued
2011
Date
2011
Author(s)
Li, Chia-Wen
Su, Ruey-Chih
Sanjaya
You, Su-Juan
Hsieh, Tsai-Hung
Chao, To-Chun
Chan, Ming-Tsair
Abstract
Ralstonia solanacearum is the causal agent of bacterial wilt (BW), one of the most important bacterial diseases worldwide. We used cDNA microarray to survey the gene expression profile in transgenic tomato (Solanum lycopersicum) overexpressing Arabidopsis (Arabidopsis thaliana) CBF1 (AtCBF1), which confers tolerance to BW. The disease-resistant phenotype is correlated with constitutive expression of the Related-to-ABI3/VP1 (RAV) transcription factor, ethylene-responsive factor (ERF) family genes, and several pathogenesis-related (PR) genes. Using a transient assay system, we show that tomato RAV2 (SlRAV2) can transactivate the reporter gene driven by the SlERF5 promoter. Virus-induced gene silencing of SlERF5 and SlRAV2 in AtCBF1 transgenic and BW-resistant cultivar Hawaii 7996 plants gave rise to plants with enhanced susceptibility to BW. Constitutive overexpression of SlRAV2 in transgenic tomato plants induced the expression of SlERF5 and PR5 genes and increased BW tolerance, while knockdown of expression of SlRAV2 inhibited SlERF5 and PR5 gene expression under pathogen infection and significantly decreased BW tolerance. In addition, transgenic tomato overexpressing SlERF5 also accumulated higher levels of PR5 transcripts and displayed better tolerance to pathogen than wild-type plants. From these results, we conclude that SlERFs may act as intermediate transcription factors between AtCBF1 and PR genes via SlRAV in tomato, which results in enhanced tolerance to BW. © 2011 American Society of Plant Biologists.
Other Subjects
Arabidopsis; Arabidopsis thaliana; Bacteria (microorganisms); Lycopersicon esculentum; Ralstonia solanacearum; Solanum; Arabidopsis protein; CBF1 protein, Arabidopsis; chlorophyll; complementary DNA; pathogenesis-related proteins, plant; transactivator protein; transcription factor; vegetable protein; Arabidopsis; disease resistance; DNA microarray; DNA sequence; gene expression; gene expression profiling; gene expression regulation; gene silencing; genetics; immunology; metabolism; microbiology; molecular genetics; nucleotide sequence; physiology; plant disease; promoter region; Ralstonia solanacearum; reporter gene; signal transduction; tomato; Arabidopsis; Arabidopsis Proteins; Base Sequence; Chlorophyll; Disease Resistance; DNA, Complementary; Gene Expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Silencing; Genes, Reporter; Lycopersicon esculentum; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Plant Diseases; Plant Proteins; Promoter Regions, Genetic; Ralstonia solanacearum; Sequence Analysis, DNA; Signal Transduction; Trans-Activators; Transcription Factors
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
33.pdf
Size
23.17 KB
Format
Adobe PDF
Checksum
(MD5):93618d9995f13a078a52844849d8a4eb